Growth and maintenance of glomerular cells under defined conditions.
Growth and maintenance of glomerular cells under defined conditions.
复制标题
肾小球细胞在规定条件下的生长和维持。
DOI：
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发表时间：
1980
影响因子：
6
通讯作者：
J. Murphy
中科院分区：
文献类型：
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作者：
T. Oberley;J. Muth;J. Murphy
在没有胎牛血清（FCS）但存在几种激素的情况下生长的肾小球不具有在血清中生长的细胞中所见的明显核周颗粒。在鸡胚提取物超滤液（CEE-U）加成纤维细胞生长因子（FGF）中生长的细胞显示出分化良好和分化不良的上皮细胞，两者均没有核周颗粒。可以在含有CEE-U加FGF加低浓度FCS的培养基中将肾小球维持数周，而没有肾小球附着或随后的细胞增殖。当这些细胞转移至30% FCS时，它们表现出介于分化良好和分化不良的上皮细胞之间的形态学特性。
Glomeruli grown in the absence of fetal calf serum (FCS) but in the presence of several hormones did not have the prominent perinuclear granules seen in cells grown in serum. Cells grown in chick embryo extract ultrafiltrate (CEE-U) plus fibroblast growth factor (FGF) showed both well-differentiated and poorly differentiated epithelial cells, neither of which had perinuclear granules. It was possible to maintain glomeruli for several weeks in media containing CEE-U plus FGF plus low concentrations of FCS without attachment of glomeruli or subsequent cell proliferation. When these cells were transferred to 30% FCS, they showed morphologic properties intermediate between those of well-differentiated and poorly differentiated epithelial cells.