Protective effect of Bauhinia tomentosa on acetic acid induced ulcerative colitis by regulating antioxidant and inflammatory mediators
Protective effect of Bauhinia tomentosa on acetic acid induced ulcerative colitis by regulating antioxidant and inflammatory mediators
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DOI：
10.1016/j.intimp.2013.03.008
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发表时间：
2013-05-01
影响因子：
5.6
通讯作者：
Guruvayoorappan, Chandrasekharan
中科院分区：
文献类型：
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作者：
Kannan, Narayanan;Guruvayoorappan, Chandrasekharan
炎症性肠病（IBD），包括克罗恩病和溃疡性结肠炎（UC），是终身和复发性胃肠道疾病，病因不明。本研究旨在评价羊蹄甲对溃疡性结肠炎（UC）的改善作用。用B处理三组动物（n = 6）。在诱导UC之前，连续5天给予毛白杨（分别为5、10、20 mg/kg B.wt），通过结肠内注射3%乙酸诱导UC。通过大体评分和组织学检查评估结肠粘膜损伤。此外，粘膜脂质过氧化物（LPO）含量，还原型谷胱甘肽（GSH），一氧化氮（NO），谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）活性证实了B。毛白杨对结肠炎有明显的抑制作用，且呈剂量依赖性。髓过氧化物酶（MPO）、肿瘤坏死因子（TNF-α）、诱导型一氧化氮合酶（iNOS）表达研究和乳酸脱氢酶（LDH）测定也支持B。毛白杨对实验性结肠炎有明显的抑制作用。其效果与标准药物柳氮磺胺吡啶相当。结肠粘膜损伤与组织学和生化指标的结果相一致。从B获得的提取物。毛白杨具有活性物质，其可能通过调节抗氧化剂和炎症介质在急性实验性结肠炎中发挥显著的保护作用。(C)2013爱思唯尔有限公司版权所有。
Inflammatory bowel diseases (IBD), including Crohn's disease and Ulcerative colitis (UC), are life-long and recurrent disorders of the gastrointestinal tract with unknown etiology. The present study is designed to evaluate the ameliorative effect of Bauhinia tomentosa during ulcerative colitis (UC). Three groups of animals (n = 6) were treated with B. tomentosa (5, 10, 20 mg/kg B.wt respectively) for 5 consecutive days before induction of UC UC was induced by intracolonic injection of 3% acetic acid. The colonic mucosal injury was assessed by macroscopic scoring and histological examination. Furthermore, the mucosal content of lipid peroxidation (LPO), reduced glutathione (GSH), nitric oxide (NO), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity confirms that B. tomentosa could significantly inhibit colitis in a dose dependent manner. The myeloperoxidase (MPO), tumor necrosis factor (TNF-alpha), inducible nitric oxide synthase (iNOS) expression studies and lactate dehydrogenase (LDH) assay also supported that B. tomentosa could significantly inhibit experimental colitis. The effect was comparable to the standard drug sulfasalazine. Colonic mucosal injury parallels with the result of histological and biochemical evaluations. The extracts obtained from B. tomentosa possess active substances, which exert marked protective effects in acute experimental colitis, possibly by regulating the antioxidant and inflammatory mediators. (C) 2013 Elsevier B.V. All rights reserved.