ULTRASTRUCTURAL OBSERVATIONS ON THE INTERACTION INVITRO BETWEEN BOVINE EOSINOPHILS AND JUVENILE FASCIOLA-HEPATICA
ULTRASTRUCTURAL OBSERVATIONS ON THE INTERACTION INVITRO BETWEEN BOVINE EOSINOPHILS AND JUVENILE FASCIOLA-HEPATICA
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DOI：
10.1017/s0031182000062703
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发表时间：
1985-01-01
期刊：
影响因子：
2.4
通讯作者：
DUFFUS, WPH
中科院分区：
文献类型：
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作者：
GLAUERT, AM;LAMMAS, DA;DUFFUS, WPH
超微结构研究表明，用特异性牛抗血清体外孵育的肝片形吸虫幼体表面有一层致密的絮状物。该材料对应于先前通过荧光显微镜表征的分泌的糖萼和牛抗体的复合物。牛嗜酸性粒细胞紧密附着在寄生虫表面没有絮状沉淀物的区域。嗜酸性粒细胞的脱颗粒进入细胞和寄生虫之间的狭窄区域。只有在这些区域才有损伤，表现为幼蛙被膜的空泡化。肝。牛嗜酸性粒细胞不能杀死幼年F.在存在特异性抗血清的情况下，寄生虫感染肝细胞是由于寄生虫表面存在由抗原/抗体复合物组成的保护层。
This ultrastructural study has shown that there is a layer of dense flocculent material on the surface of juvenile Fasciola hepatica incubated in vitro with specific bovine antiserum. This material corresponds to the complexes of secreted glycocalyx and bovine antibody previously characterized by fluorescence microscopy. Bovine eosinophils attach closely to those regions of the parasite''s surface that are free of flocculent precipitates. This close attachment is followed by degranulation of the eosinophils into the narrow zone between the cells and the parasite. Only in these regions is damage, in the form of vacuolation of the tegument seen within the juvenile F. hepatica. It is concluded that the inability of bovine eosinophils to kill juvenile F. hepatica in the presence of specific antiserum results from the presence of a protective layer, consisting of antigen/antibody complexes, on the parasite''s surface.