Role of MinD-membrane association in Min protein interactions.
Role of MinD-membrane association in Min protein interactions.
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MinD 膜关联在 Min 蛋白相互作用中的作用。
DOI：
10.1128/jb.188.8.2993-3001.2006
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Rothfield,Lawrence
中科院分区：
文献类型：
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作者：
Taghbalout,Aziz;Ma,Luyan;Rothfield,Lawrence
在大肠杆菌中分裂位点的放置涉及MinD蛋白与MinC和MinE以及与其他MinD分子的相互作用，以形成膜相关的聚合物结构。在这项工作中，作为这些相互作用的研究的一部分，我们建立了异源膜相关蛋白，如MinD可以靶向酵母核膜，仅依赖于膜结合结构域和核靶向序列的存在。使用细胞色素B5的内在MinD膜靶向结构域或完全无关的膜靶向结构域，靶向核膜同样有效。然后使用酵母双杂交系统，将其膜靶向序列不同的嵌合蛋白用于建立MinD相互作用中膜缔合和膜锚特异性的作用。嵌合蛋白也被用于显示MinD和MinE在E中的膜结合。大肠杆菌细胞对膜锚没有特异性，而MinDE极区和MinE环的形成需要天然MinD膜靶向序列的存在。
Division site placement inEscherichia coliinvolves interactions of the MinD protein with MinC and MinE and with other MinD molecules to form membrane-associated polymeric structures. In this work, as part of a study of these interactions, we established that heterologous membrane-associated proteins such as MinD can be targeted to the yeast nuclear membrane, dependent only on the presence of a membrane-binding domain and a nuclear targeting sequence. Targeting to the nuclear membrane was equally effective using the intrinsic MinD membrane-targeting domain or the completely unrelated membrane-targeting domain of cytochromeb5. The chimeric proteins differing in their membrane-targeting sequences were then used to establish the roles of membrane association and specificity of the membrane anchor in MinD interactions, using the yeast two-hybrid system. The chimeric proteins were also used to show that the membrane association of MinD and MinE inE. colicells had no specificity for the membrane anchor, whereas formation of MinDE polar zones and MinE rings required the presence of the native MinD membrane-targeting sequence.