GENETIC-RELATIONSHIPS DETERMINED BY A DNA HETERODUPLEX MOBILITY ASSAY - ANALYSIS OF HIV-1 ENV GENES
GENETIC-RELATIONSHIPS DETERMINED BY A DNA HETERODUPLEX MOBILITY ASSAY - ANALYSIS OF HIV-1 ENV GENES
复制标题
DOI：
10.1126/science.8235655
复制
发表时间：
1993-11-19
期刊：
影响因子：
56.9
通讯作者：
MULLINS, JI
中科院分区：
文献类型：
--
作者：
DELWART, EL;SHPAER, EG;MULLINS, JI
人类免疫缺陷病毒（HIV）的遗传多样性被认为是影响免疫逃逸和最终疫苗效力的一个主要问题。在此，基于观察到相关序列之间形成的DNA异双链在聚丙烯酰胺凝胶中的迁移率与它们的分化程度成正比，描述了用于检测和估计HIV毒株之间遗传差异的简单快速方法。可靠的系统发育亚型被分配给来自世界各地的HIV-1毒株。当病毒来源于相同或流行病学上相关的个体时，它们之间的关系最为密切。当来自流行病学上无关联的个体时，特定地理区域的病毒之间的关系与在人群中检测到HIV-1的时间长度和引起广泛感染的毒株数量相关。因此，异双工迁移分析提供了一种通过协助艾滋病毒分类来加快流行病学调查的工具，并且很容易适用于其他传染因子和细胞基因的筛选和表征。
The genetic diversity of human immunodeficiency virus (HIV) is a major concern thought to impact on immunologic escape and eventual vaccine efficacy. Here, simple and rapid methods are described for the detection and estimation of genetic divergence between HIV strains on the basis of the observation that DNA heteroduplexes formed between related sequences have a reduced mobility in polyacrylamide gels proportional to their degree of divergence. Reliable phylogenetic subtypes were assigned for HIV-1 strains from around the world. Relationships between viruses were closest when derived from the same or epidemiologically linked individuals. When derived from epidemiologically unlinked individuals, the relationships between viruses in a given geographic region correlated with the length of time HIV-1 had been detected in the population and the number of strains initiating widespread infection. Heteroduplex mobility analysis thus provides a tool to expedite epidemiological investigations by assisting in the classification of HIV and is readily applicable to the screening and characterization of other infectious agents and cellular genes.