Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma
Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma
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碱性转录因子3在鼻咽癌中的表达及临床意义
DOI：
10.3892/ol.2018.9699
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发表时间：
2019-01-01
期刊：
影响因子：
2.9
通讯作者：
Huang, Zhigang
中科院分区：
文献类型：
--
作者：
Chen, Ping;Zhong, Qi;Huang, Zhigang
碱性转录因子3（BTF 3）是一种转录因子和凋亡调节因子，在肿瘤中差异表达。为了进一步了解BTF 3在鼻咽癌中的作用，本研究分析了BTF 3在鼻咽癌中的表达及其对细胞功能的影响。采用逆转录-定量聚合酶链反应和免疫组织化学方法分析了46例鼻咽癌组织和46例癌旁正常组织中BTF 3的转录率。通过特异性小干扰RNA分子进行NPC细胞中的BTF 3沉默。使用Cellomic测定系统通过增殖测定和集落形成测定分析BTF 3的功能。BTF 3在癌组织中的阳性表达率明显高于癌旁组织（P
Basic transcription factor 3 (BTF3), a transcription factor and modulator of apoptosis, is differentially expressed in carcinoma. To acquire further understanding of the involvement of BTF3 in carcinoma, the present study analyzed the expression of BTF3, as well as its role in cell function in nasopharyngeal carcinoma (NPC). BTF3 transcription rates in human NPC samples (n=46) and adjacent normal tissue samples (n=46) were analyzed using reverse transcription-quantitative polymerase chain reaction and immunohistochemistry. BTF3-silencing in NPC cells was performed via specific small interfering RNA molecules. The function of BTF3 was analyzed by proliferation assays and colony forming assays using a Cellomic assay system. The positive expression rates of BTF3 were significantly increased in cancerous tissues compared with those in adjacent tissues (P