Hemoglobin self-assembly and antibacterial activities of bio-modified Ag-MgO nanocomposites by different concentrations of Artemisia haussknechtii and Protoparmeliopsis muralis extracts
Hemoglobin self-assembly and antibacterial activities of bio-modified Ag-MgO nanocomposites by different concentrations of Artemisia haussknechtii and Protoparmeliopsis muralis extracts
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DOI：
10.1016/j.ijbiomac.2019.10.207
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发表时间：
2020-06-01
影响因子：
8.2
通讯作者：
Karimi, Naser
中科院分区：
文献类型：
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作者：
Alavi, Mehran;Karimi, Naser
金属纳米颗粒和金属纳米复合材料与血液蛋白的相互作用从细胞毒性和生产新的药物传递系统的角度来看是重要的。在本研究中，采用不同浓度的蒿属药用植物和乡土原生地衣提取物对Ag-MgO纳米复合材料进行生物修饰。采用紫外-可见光谱、扫描电镜（SEM）、x射线粉末衍射（XRD）和傅里叶变换红外（FT-IR）等方法对NCs的理化性质进行了表征。采用碟形扩散法和最低抑菌/杀菌浓度法（MIC和MBC）测定了NCs对耐甲氧西林金黄色葡萄球菌（MRSA）、大肠杆菌ATCC 25922和铜绿假单胞菌ATCC 27853的抑菌效果。此外，在最后一节中，生物修饰的nc （bnc）与血红蛋白（Hb）蛋白的相互作用通过SEM，原子力显微镜（AFM）和FT-IR技术进行了研究。植物提取物（10%w/v）对大肠杆菌ATCC 25922的抑菌效果最好，抑菌带直径（IZD）为20 +/- 0.89 mm。上述分析证实Hb在bnc周围聚集。本研究说明了包括硫醇和咪唑基团在内的各种官能团在bnc周围Hb自组装中的作用。(C) 2019 Elsevier B.V.版权所有
Interaction of metal nanoparticles and metal nanocomposites with blood proteins is important from the perspectives of cytotoxicity and production of novel drug delivery systems. In this study, Ag-MgO nanocomposites were bio-modified by different concentrations of Artemisia haussknechtii medicinal plant and Protoparmeliopsis muralis medicinal lichen extracts. Determination of physicochemical properties of NCs were carried out by UV-Visible spectroscopy, scanning electron microscope (SEM), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR) analyses. Antibacterial effects of NCs against methicillin-resistant Staphylococcus aureus (MRSA), E. coli ATCC 25922, and P. aeruginosa ATCC 27853 bacteria were measured by disc diffusion and minimum inhibition/bactericidal concentrations (MIC and MBC). Also, in final section, interaction of bio-modified NCs (BNCs) with hemoglobin (Hb) protein was surveyed by SEM, atomic force microscope (AFM), and FT-IR techniques. BNCs by plant extract (10%w/v) showed highest antibacterial effects on E. coli ATCC 25922 as inhibition zone diameter of (IZD) value of 20 +/- 0.89 mm. Aggregation of Hb around BNCs was approved by mentioned analyses. This investigation illustrated contribution of various functional groups including thiol and imidazole groups in self-assembly of Hb surrounding BNCs. (C) 2019 Elsevier B.V. All rights reserved.