Protein detecting arrays based on cationic polythiophene-DNA-aptamer complexes
Protein detecting arrays based on cationic polythiophene-DNA-aptamer complexes
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DOI：
10.1002/adma.200601651
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发表时间：
2006-10-17
影响因子：
29.4
通讯作者：
Leclerc, Mario
中科院分区：
文献类型：
--
作者：
Aberem, Maite Bera;Najari, Ahmed;Leclerc, Mario
带有阳离子聚噻吩光学传感器的适配体，可以在不到一小时的时间内在阿托摩尔范围内的微阵列上特异性检测人凝血酶，而无需对目标进行任何标记。该系统可以被修改并用作检测各种蛋白质或其他生物分子的探针。这项工作为基因组学和蛋白质组学中简单快速的多参数分析开辟了新的有趣的可能性。
aptamer with a cationic polythiophene optical transducer, human thrombin can be specifically detected on microarrays in the attomole range in less than one hour without any tagging of the target. The system can be modified and utilized as a probe for the detection of various proteins or other biomolecules. This work opens new interesting possibilities for simple and rapid multiparametric analysis in genomics and proteomics.