Effect of sterilisation by gamma irradiation on the ability of polycaprolactone (PCL) to act as a scaffold material
Effect of sterilisation by gamma irradiation on the ability of polycaprolactone (PCL) to act as a scaffold material
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DOI：
10.1016/j.medengphy.2008.07.005
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发表时间：
2009-03-01
影响因子：
2.2
通讯作者：
Jenkins, Mike J.
中科院分区：
文献类型：
--
作者：
Cottam, Emily;Hukins, David W. L.;Jenkins, Mike J.
本文研究了伽马射线灭菌（剂量 2.5 Mrad）对聚己内酯 (PCL) 以下特性的影响：(1) 降解率（由脂肪酶催化），(2) 机械特性，(3) 细胞在其表面附着并随后生长的能力。凝胶渗透色谱（GPC）用于测定伽马射线照射对重均（M（w））和数均（M（n））分子量的影响。伽马射线照射显着降低了降解速率，尽管降解速率取决于聚合物的初始质量；当通过扫描电子显微镜检查时，它还会影响降解样品的外观。辐照还显着增加了 PCL 的机械屈服应力，但没有增加失效应力。它导致 M(w) 显着增加和 M(n) 减少，这可能归因于断链和交联。软骨细胞在 PCL 上的附着和生长不受伽马射线照射的显着影响。 (C) 2008 年 IPEM。由爱思唯尔有限公司出版。保留所有权利。
This paper investigates the effect of sterilisation by gamma irradiation (dose 2.5 Mrad) on the following properties of polycaprolactone (PCL): (1) degradation rate (catalysed by lipase), (2) mechanical properties, (3) the ability of cells to attach and subsequently grow on its surface. Gel permeation chromatography (GPC) was used to determine the effects of gamma irradiation of weight average (M(w)) and number average (M(n)) molecular weights. Gamma irradiation significantly decreased the rate of degradation, although the rates depended on the initial mass of polymer; it also affected the appearance of the degraded specimens when they were examined by scanning electron microscopy. Irradiation also significantly increased the mechanical yield stress but not the failure stress of PCL. It caused a significant increase in M(w) and decrease in M(n) that could be attributed to chain scission and cross-linking. Chondrocyte attachment and growth on PCL was not significantly affected by gamma irradiation. (C) 2008 IPEM. Published by Elsevier Ltd. All rights reserved.