ISOLATION OF PLACENTAL CADHERIN CDNA - IDENTIFICATION OF A NOVEL GENE FAMILY OF CELL CELL-ADHESION MOLECULES
ISOLATION OF PLACENTAL CADHERIN CDNA - IDENTIFICATION OF A NOVEL GENE FAMILY OF CELL CELL-ADHESION MOLECULES
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DOI：
10.1002/j.1460-2075.1987.tb02698.x
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发表时间：
1987-12-01
期刊：
影响因子：
11.4
通讯作者：
TAKEICHI, M
中科院分区：
文献类型：
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作者：
NOSE, A;NAGAFUCHI, A;TAKEICHI, M
Ca2+依赖性细胞间粘附分子（称为钙粘蛋白）被分为具有不同组织分布和不同细胞间结合特异性的亚类。我们报道了编码存在于胎盘中的钙粘蛋白（称为 P-钙粘蛋白）的 cDNA 的克隆。推导的序列编码822个氨基酸的多肽，具有完整膜蛋白的特征。对 P-钙粘蛋白与其自身的氨基酸序列同源性的计算机搜索表明，该分子在胞外结构域中含有内部重复序列。 P-钙粘蛋白与上皮钙粘蛋白（E-钙粘蛋白）一级结构的比较表明，它们的氨基酸序列有58%的同源性。这些结果为我们的假设提供了证据，即钙粘蛋白构成一个基因家族。
Ca2+-dependent cell-cell adhesion molecules, termed cadherins, are classified into subclasses with different tissue distributions and distinct cell-cell binding specificities. We report the cloning of cDNA encoding a cadherin present in the placenta which is called P-cadherin. The deduced sequence encodes a polypeptide of 822 amino acids with the characteristic features of integral membrane proteins. A computer search of the amino acid sequence homology of P-cadherin against itself showed that this molecule contains internal repeats in the extracellular domain. Comparison of the primary structure of P-cadherin with that of the epithelial cadherin (E-cadherin) showed that there is 58% homology in their amino acid sequences. These results provide evidence for our hypothesis that cadherins constitute a gene family.