Differences in delta opioid receptor antinociception, binding, and mRNA levels between BALB/c and CXBK mice.
Differences in delta opioid receptor antinociception, binding, and mRNA levels between BALB/c and CXBK mice.
复制标题
BALB/c 和 CXBK 小鼠之间 δ 阿片受体镇痛、结合和 mRNA 水平的差异。
DOI：
10.1016/s0006-8993(98)00696-9
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发表时间：
1998
期刊：
影响因子：
2.9
通讯作者：
Inturrisi,CE
中科院分区：
文献类型：
--
作者：
Kest,B;Beczkowska,I;Franklin,SO;Lee,CE;Mogil,JS;Inturrisi,CE
Mu 和 delta 阿片受体已被证明可介导脊髓上阿片类镇痛作用。尽管重组近交系 CXBK 小鼠在 mu 阿片受体抗伤害作用、结合密度和 mRNA (MOR-1) 水平方面明显缺乏，但对该品系中 delta 阿片受体过程知之甚少。因此，本研究比较了 CXBK 小鼠及其 BALB/c 品系祖先的 δ 阿片类镇痛作用、全脑受体结合水平和 mRNA (DOR-1) 水平。分别脑室内注射选择性 delta1 和 delta2 阿片类药物 DPDPE 和 [d-Ala2]deltorphin II 后，CXBK 小鼠在甩尾试验中表现出相对较低的镇痛作用，导致该品系中两种激动剂的 ED50 值显着增加。在 CXBK 小鼠中也观察到 [3H][d-Ala2]deltorphin II 的全脑特异性结合减少，但 [3H]DPDPE 没有减少。与 DOR-1 探针的溶液杂交揭示了该菌株尾壳核、额叶皮层和脊髓的转录水平增加。目前的数据表明，CXBK 小鼠中 delta1 和 delta2 阿片类药物抗伤害作用缺乏，同时伴有全脑 delta2 受体结合减少和 DOR-1 区域增加。这些观察结果是否存在因果关系仍有待澄清。
Mu and delta opioid receptors have been demonstrated to mediate supraspinal opioid antinociception. Whereas the recombinant inbred CXBK mouse is notably deficient in mu opioid receptor antinociception, binding density, and mRNA (MOR-1) levels, little is known about delta opioid receptor processes in this strain. The present study thus compared CXBK mice and their BALB/c strain progenitors with respect to delta opioid antinociception, whole-brain receptor binding levels, and mRNA (DOR-1) levels. Following intracerebroventricular injections of the selective delta1and delta2opioids DPDPE and [d-Ala2]deltorphin II, respectively, CXBK mice displayed relatively lower antinociception on the tail-flick test, resulting in significantly increased ED50values for both agonists in this strain. Decreased whole-brain specific binding of [3H ][d-Ala2]deltorphin II, but not [3H ]DPDPE, was also observed in CXBK mice. Solution hybridization with a probe for the DOR-1 revealed increased transcript levels in the caudate-putamen, frontal cortex, and spinal cord of this strain. The present data demonstrate a deficiency in delta1and delta2opioid antinociception in CXBK mice concomitant with reductions in whole-brain delta2receptor binding and regional increases in DOR-1. Whether these observations are causally related remains to be clarified.