Evolutionary dynamics of triosephosphate isomerase gene intron location pattern in Metazoa: A new perspective on intron evolution in animals
Evolutionary dynamics of triosephosphate isomerase gene intron location pattern in Metazoa: A new perspective on intron evolution in animals
复制标题
后生动物中磷酸三糖异构酶基因内含子定位模式的进化动力学：动物内含子进化的新视角。
DOI：
10.1016/j.gene.2016.11.027
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发表时间：
2017-02-20
期刊：
影响因子：
3.5
通讯作者：
Wen，Jianfan
中科院分区：
文献类型：
--
作者：
Chen，Bing;Shao，Jingru;Wen，Jianfan
内含子的进化，包括其在真核生物的进化转型和多样化的动力学，仍然是难以捉摸的。由于数据不足，系统发育框架不明确，以及不适当的外群应用，分类单元采样不足可能是原因之一。此外，基因内所有内含子的完整性以前常常被忽视。利用磷酸丙糖异构酶基因（tim）的古老保守性、后生动物相对稳健的进化史以及领鞭类作为外类群的优势，研究了后生动物timintron location pattern（ILP）的进化动力学。从10门133种代表性植物中鉴定出30种类型的ILP。最常见的一个内含子是后生动物的共同祖先ILP，它几乎在原生动物祖先中就已形成，并被保留下来，一直传递到后生动物门的各个祖先。在随后的动物多样化，它经历了不同的进化轨迹：在Deuterostomia，它几乎完全保留，只有在几个物种的变化与相对较近的快速发展的历史，而在快速辐射Protostomia，除了少数但显着的保留，它通常显示广泛的内含子损失和一些收益。因此，一个共同的祖先的外显子-内含子排列模式的动物基因是肯定的，除了被证实的早期动物基因的“内含子丰富的观点”，新的见解，高外显子-内含子重排的基因似乎与最近的快速进化的谱系/物种/基因组，但没有相关的古代动物进化中的主要进化转变，被揭示。
Intron evolution, including its dynamics in the evolutionary transitions and diversification of eukaryotes, remains elusive. Inadequate taxon sampling due to data shortage, unclear phylogenetic framework, and inappropriate outgroup application might be among the causes. Besides, the integrity of all the introns within a gene was often neglected previously. Taking advantage of the ancient conserved triosephosphate isomerase gene (tim), the relatively robust phylogeny of Metazoa, and choanoflagellates as outgroup, the evolutionary dynamics oftimintron location pattern (ILP) in Metazoa was investigated. From 133 representative species of ten phyla, 30 types of ILPs were identified. A most common one, which harbors the maximum six intron positions, is deduced to be the common ancestraltimILP of Metazoa, which almost had formed in their protozoan ancestor and was surprisingly retained and passed down till to each ancestors of metazoan phyla. In the subsequent animal diversification, it underwent different evolutionary trajectories: within Deuterostomia, it was almost completely retained only with changes in a few species with relatively recently fast-evolving histories, while within the rapidly radiating Protostomia, besides few but remarkable retention, it usually displayed extensive intron losses and a few gains. Therefore, a common ancestral exon-intron arrangement pattern of an animal gene is definitely discovered; besides the ‘intron-rich view’ of early animal genes being confirmed, the novel insight that high exon-intron re-arrangements of genes seem to be associated with the relatively recently rapid evolution of lineages/species/genomes but have no correlation with the ancient major evolutionary transitions in animal evolution, is revealed.