The bovine leukemia virus p34 is a transactivator protein.
The bovine leukemia virus p34 is a transactivator protein.
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牛白血病病毒 p34 是一种反式激活蛋白。
DOI：
10.1002/j.1460-2075.1987.tb02661.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
J. Ghysdael
中科院分区：
文献类型：
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作者：
Lucas Willems;A. Gégonne;Guangling Chen;A. Burny;R. Kettmann;J. Ghysdael
构建了含有BLV包膜后长读码框的重组Moloney小鼠逆转录病毒，并将其导入pSi2包装细胞系。它们编码并表达一种34kd的蛋白，能够在各自的转基因细胞中反式激活BLV长末端重复序列导向的基因表达。这些数据表明，BLV X-Lor基因编码一个p34反式激活产物。此外，不同的细胞系产生了传染性重组逆转录病毒，能够将X-Lor基因转移到受体细胞中。BLV反式激活蛋白的可获得性应该使我们能够理解反式激活蛋白在BLV诱导的白血病发生中的作用。
Recombinant Moloney murine retroviruses containing the BLV post‐envelope long open reading frame were constructed and transfected into the psi 2 packaging cell line. They were shown to encode and to express a 34‐kd protein able to transactivate the BLV long terminal repeat‐directed gene expression in the respective transfected cells. These data demonstrate that the BLV X‐LOR gene encodes a p34 transactivator product. Furthermore, the different cell lines produced infectious recombinant retroviruses capable of transferring X‐LOR genes into recipient cells. The availability of the BLV transactivator protein should allow us to understand the role of the transactivator protein in BLV‐induced leukemogenesis.