A PRAGMATIC APPROACH TO THE ANALYSIS OF DNA HISTOGRAMS WITH A DEFINABLE G1 PEAK
A PRAGMATIC APPROACH TO THE ANALYSIS OF DNA HISTOGRAMS WITH A DEFINABLE G1 PEAK
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DOI：
10.1002/cyto.990080101
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发表时间：
1987-01-01
期刊：
影响因子：
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通讯作者：
SMITH, PJ
中科院分区：
文献类型：
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作者：
WATSON, JV;CHAMBERS, SH;SMITH, PJ
描述了一种DNA直方图分析方法，该方法仅依赖于数据服从正态分布的简单假设和存在G1峰的要求。基于从整个直方图中提取S相分量的假设，推导出一个概率密度函数。用模拟数据对模型进行了检验，发现G1、S和G1+M的预测比例与已知比例具有较好的一致性。实验数据的副本之间也发现了很好的一致性。在某些类型的直方图的分析中存在一些系统误差。然而，与生物和实验变化相比，这些导致的误差很小，并且低于当前使用的算法的平均水平。程序只需要两个排队的请求，即要在其上执行分析的开始和结束通道的请求。这些算法在只有28K可寻址存储器的微型计算机上快速执行。在350多次分析中，只有两次失败，该方法既可以用于受药物和辐射干扰的人群，也可以用于未受干扰的人群。
A method for DNA histogram analysis is described that depends only on the simple assumption that the data are normally distributed and a requirement that a G1 peak is present. A probability density function was derived from the assumption that extracted the S-phase component from the whole histogram. The model was tested with simulated data, and good agreement between predicted and known proportions in G1, S, and G1 + M was found. Good agreement was also found between duplicates of experimentally derived data. Some systematic errors are present in the analysis of certain types of histograms. However, these result in small errors when compared with biological and experimental variation and are less than the average of algorithms in current use. The program required only two queued requests, those of the start and the end channels over which the analysis is to be performed. The algorithms perform rapidly on a microcomputer with only 28K addressable memory. Only two failures occurred in over 350 analyses and the method can be used for drug- and radiation-perturbed populations as well as with unperturbed.