Distribution, characterization and clinical significance of microglia in glioneuronal tumours from patients with chronic intractable epilepsy
Distribution, characterization and clinical significance of microglia in glioneuronal tumours from patients with chronic intractable epilepsy
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DOI：
10.1111/j.1365-2990.2004.00636.x
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发表时间：
2005-06-01
影响因子：
5
通讯作者：
Troost, D
中科院分区：
文献类型：
--
作者：
Aronica, E;Gorter, JA;Troost, D
小胶质细胞/巨噬细胞系的细胞是不同脑肿瘤的重要组成部分。然而，关于小胶质细胞/巨噬细胞系统在胶质神经性肿瘤中的作用及其对这些病变高度致痫的可能作用的信息很少。本文用免疫细胞化学方法研究了CD68和人类白细胞抗原-DR在慢性难治性癫痫患者的神经胶质瘤(GG，n=30)和胚胎发育不良神经上皮肿瘤(DNT，n=17)中的分布。在绝大多数神经胶质瘤中可见大量的小胶质细胞/巨噬细胞，包括肿瘤内和瘤周区域。HLA-DR阳性的活化小胶质细胞定位于血管周围，并聚集在肿瘤神经细胞周围。激活的小胶质细胞密度与癫痫的持续时间以及手术切除前癫痫发作的频率相关。这些观察表明，小胶质/巨噬细胞系统细胞的存在是神经胶质瘤的一个特征，在功能上与癫痫有关，无论是在癫痫的发生中直接还是通过癫痫活动后的激活。
Cells of the microglia/macrophage lineage represent an important component of different brain tumours. However, there is little information about the microglia/macrophage cell system in glioneuronal tumours and its possible contribution to the high epileptogenecity of these lesions. In the present study, the distribution of cells of the microglia/macrophage lineage was studied by immunocytochemistry for CD68 and human leucocyte antigen (HLA)-DR in a group of glioneuronal tumours, including gangliogliomas (GG, n = 30), and dysembryoplastic neuroepithelial tumours (DNT, n = 17), from patients with chronic intractable epilepsy. A significant number of microglia/macrophage cells were observed in the large majority of glioneuronal tumours, both within the tumour and in the peritumoral region. Activated microglial cells positive for HLA-DR were localized around blood vessels and clustered around tumour neuronal cells. The density of activated microglial cells correlated with the duration of epilepsy, as well as with the frequency of seizures prior to surgical resection. These observations indicate that the presence of cells of the microglial/macrophage cell system is a feature of glioneuronal tumours and is functionally related to epilepsy, either directly in epileptogenesis or through activation following seizure activity.