DIFFERENTIAL ROLES FOR 3 CONSERVED HISTIDINE-RESIDUES WITHIN THE LARGE SUBUNIT OF CARBAMOYL PHOSPHATE SYNTHETASE
DIFFERENTIAL ROLES FOR 3 CONSERVED HISTIDINE-RESIDUES WITHIN THE LARGE SUBUNIT OF CARBAMOYL PHOSPHATE SYNTHETASE
复制标题
DOI：
10.1021/bi00052a030
复制
发表时间：
1993-01-12
期刊：
影响因子：
2.9
通讯作者：
RAUSHEL, FM
中科院分区：
文献类型：
--
作者：
MILES, BW;MAREYA, SM;RAUSHEL, FM
通过序列一致性比较，从大肠杆菌中鉴定出位于磷酸氨基甲酰合成酶大亚基中的3个保守组氨酸残基His-243、His-781和His-788。这三个组氨酸残基分别突变为天冬酰胺残基。研究发现，H243N突变体酶对合成氨甲酰磷酸至关重要，因为突变体蛋白不能以显著的速率合成氨甲酰磷酸(
Three conserved histidine residues, His-243, His-781, and His-788, located within the large subunit of carbamoyl phosphate synthetase from Escherichia coli were identified by sequence identity comparisons. These three histidine residues were individually mutated to asparagine residues. The H243N mutant enzyme was found to bc critical for carbamoyl phosphate synthesis as the mutant protein was unable to synthesize carbamoyl phosphate at a significant rate (