Effectiveness of chemical agents in removing platinum from DNA isolated from cisplatin-treated HL-60 cells.
Effectiveness of chemical agents in removing platinum from DNA isolated from cisplatin-treated HL-60 cells.
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发表时间：
1992
影响因子：
1.7
通讯作者：
R. Oliński;R. Briggs;M. Basinger;M. Jones
中科院分区：
文献类型：
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作者：
R. Oliński;R. Briggs;M. Basinger;M. Jones
人早幼粒细胞白血病细胞HL-60用顺铂[顺式二氨二氯铂（II）]（2 mM，1 h）处理。分离DNA-顺铂-蛋白质复合物并暴露于硫脲（1 M）、NaCN（100 mM）、二乙基二硫代氨基甲酸酯（500 mM）或N-甲基-D-葡糖胺二硫代氨基甲酸酯（500 mM）12小时。通过原子吸收光谱法测量铂的释放。氰化钠是最有效的试剂，释放约90%的DNA结合铂。噻托溴铵是效果最差的药剂，而二硫代氨基甲酸酯则表现出中间体。还评价了同一组试剂从蛋白质-顺铂-DNA复合物中分离蛋白质的能力，类似地，二硫代氨基甲酸酯也是最有效的。
Human promyelocytic leukemia cells, HL-60, were treated with cisplatin [cis-diamminedichloroplatinum (II)] (2 mM, 1 h). DNA-cisplatin-protein complexes were isolated and exposed to thiourea (1 M), NaCN (100 mM), diethyldithio-carbamate (500 mM), or N-methyl-D-glucamine dithiocarbamate (500 mM) for 12 h. The release of platinum was measured by atomic absorption spectroscopy. Sodium cyanide was the most effective agent, releasing about 90% of the DNA-bound platinum. Thiourea was the least effective agent, while dithiocarbamates exhibited an intermediate. The ability of the same group of agents to split the proteins off from the protein-cisplatin-DNA complex was also evaluated and similarly dithiocarbamate were also the most effective.