Differential cyclooxygenase-1 and -2 gene expression in human myometria from preterm and term deliveries.
Differential cyclooxygenase-1 and -2 gene expression in human myometria from preterm and term deliveries.
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DOI：
10.1210/jcem.79.3.8077379
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发表时间：
1994-09
期刊：
影响因子：
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通讯作者：
J. Zuo;Z M Lei;C V Rao;M. Pietrantoni;V D Cook
中科院分区：
文献类型：
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作者：
J. Zuo;Z M Lei;C V Rao;M. Pietrantoni;V D Cook
采用原位杂交、免疫印迹和免疫细胞化学方法研究了环氧化酶-1（考克斯-1）和考克斯-2基因在早产和足月妊娠子宫肌层中的表达。原位杂交显示子宫平滑肌同时含有考克斯-1和考克斯-2信使核糖核酸转录本。免疫印迹显示子宫肌层含有55和67千道尔顿的考克斯-1蛋白以及72和56千道尔顿的考克斯-2蛋白。免疫细胞化学显示考克斯-1和考克斯-2蛋白存在于子宫肌层平滑肌中。考克斯-1和考克斯-2基因在子宫肌瘤中的表达存在差异。例如，虽然考克斯-1表达较低，但与未分娩的早产相比，足月时考克斯-2表达较高。此外，尽管考克斯-1的表达不依赖于劳动，考克斯-2的表达是较低的劳动相比，没有在劳动早产和足月妊娠。子宫肌层平滑肌也对前列腺素E2（PGE 2）和PGF 2 α进行免疫染色，表明环加氧酶具有催化活性。PGE 2和PGF 2 α的变化与考克斯-1酶的变化一致，即足月妊娠时低于早产，与考克斯-2酶的变化一致，即早产和足月妊娠分娩时低于早产。总之，我们的研究结果表明，怀孕的人子宫肌层表达考克斯-1和考克斯-2基因。这些基因的表达在早产或足月妊娠子宫肌瘤中与分娩中不同，而在分娩中没有。
The expression of cyclooxygenase-1 (COX-1) and COX-2 genes in myometria from preterm and term pregnancies was investigated by in situ hybridization, immunoblotting, and immunocytochemistry. In situ hybridization revealed that myometrial smooth muscle contained both COX-1 and COX-2 messenger ribonucleic acid transcripts. Immunoblotting revealed that myometria contained 55- and 67-kilodalton COX-1 proteins as well as 72- and 56-kilodalton COX-2 proteins. Immunocytochemistry showed that COX-1 and COX-2 proteins are present in the myometrial smooth muscle. The expression of COX-1 and COX-2 genes differed in myometria. For example, although COX-1 expression was lower, COX-2 expression was higher at term compared to preterm pregnancy not in labor. In addition, although COX-1 expression was not dependent on labor, COX-2 expression was lower with labor compared to not in labor for both preterm and term pregnancy. The myometrial smooth muscle also immunostained for prostaglandin E2 (PGE2) and PGF2 alpha, suggesting that the cyclooxygenases are catalytically active. The changes in PGE2 and PGF2 alpha paralleled the changes in COX-1 enzyme in terms of being lower at term compared to preterm pregnancy and the changes in COX-2 enzyme in terms of being lower during labor at preterm and term pregnancy. In summary, our results demonstrate that pregnant human myometria express both COX-1 and COX-2 genes. The expression of these genes differed in preterm or term pregnancy myometria from in labor and not in labor.