Rapid identification of glycerophospholipids from RAW264.7 cells by UPLC/ESI -QTOF-MS.
Rapid identification of glycerophospholipids from RAW264.7 cells by UPLC/ESI -QTOF-MS.
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DOI：
10.1002/bmc.3216
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发表时间：
2014-12
期刊：
影响因子：
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通讯作者：
Yuqi She;J. Song;E. Yang;Lilii Zhao;Yan-Mei Zhong;W. Rui;Y. Feng;Xia Wu
中科院分区：
文献类型：
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作者：
Yuqi She;J. Song;E. Yang;Lilii Zhao;Yan-Mei Zhong;W. Rui;Y. Feng;Xia Wu
本研究旨在建立一种快速、灵敏的超高效液相色谱-电喷雾电离四次飞行时间串联质谱仪(UPLC/Q-TOF-MS)分析方法，用于鉴定RAW264.7细胞中的甘油磷脂(GPL)。共鉴定出78种GPL，包括22种磷脂酰乙醇胺(PES)、49种磷脂酰胆碱(PC)、4种磷脂酰甘油、1种磷脂酰肌醇和2种未知的GPL。首次鉴定为PC(14：0/16：1)、PC(14：0/16：0)、PE(0：0/20：3)、PE(22：5/0：0)和PE(22：3/0：0)。UPLC/Q-TOF-MS方法适用于RAW264.7细胞中GPLs的靶向分析，有助于我们发现与炎症性疾病相关的新的GPLs成分，并解释其在炎症过程中的药理作用。
The study aims to develop a rapid, sensitive ultra-performance liquid chromatography coupled with an electrospray ionization quadruple time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS) analytical method for identifying glycerophospholipids (GPLs) from RAW264.7 cells. A total of 78 GPLs including 22 phosphatidylethanolamines (PEs), 49 phosphatidylcholines (PCs), four phosphatidylglycerols, one phosphatidylinositol and two unknown GPLs were identified. PC (14:0/16:1), PC (14:0/16:0), PE (0:0/20:3), PE (22:5/0:0) and PE (22:3/0:0) were identified for the first time. The UPLC/Q-TOF-MS method is suitable for targeting analysis of GPLs from RAW264.7 cells, which allows us to find out new GPLs compositions related to inflammatory diseases and to explain their pharmacological roles in inflammatory process.