Molecular identification of individual oak and fir trees from maternal tissues of their fruits or seeds
Molecular identification of individual oak and fir trees from maternal tissues of their fruits or seeds
复制标题
从果实或种子的母体组织中对单个橡树和冷杉树进行分子鉴定
DOI：
10.1007/s00468-002-0244-9
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
M. Fladung
中科院分区：
文献类型：
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作者：
B. Ziegenhagen;S. Liepelt;V. Kuhlenkamp;M. Fladung
DNA标记对纯母体组织的适用性在树木中几乎没有得到解决。我们一直专注于非薄壁母组织的果实和种子的有梗橡树（Quercus robur L。）和银冷杉（Abies albaMill.）并研究了它们是否可用于母树的直接分子鉴定。从橡子的单个木质果皮以及银种子的单个干翅中提取了足够数量和质量的总DNA。在高度多态性的微卫星位点通过PCR分析DNA。果皮和翅膀的多位点基因型与各自的母树的比较显示出绝对的同一性。因此，母树可以通过对它们的果实或种子进行基因分型来识别。结果表明，DNA指纹的适用性木本和/或干燥的种子组织没有破坏胚和胚乳或显着的污染。现在预计在扩散生物学以及法医学和森林管理方面将取得进展。
The applicability of DNA markers to purely maternal tissues has been scarcely addressed in trees. We have focused on non-parenchymatic maternal tissues of the fruits and seeds of pedunculate oak (Quercus roburL.) and silver fir (Abies albaMill.) and investigated whether they can be used for a direct molecular identification of the mother trees. Total DNA with sufficient quantity and quality was extracted from single woody pericarps of acorns as well as from single dry wings of silver fir seeds. The DNA was analysed by PCR at highly polymorphic microsatellite loci. A comparison of the multi-locus genotypes from pericarps and wings with those of the respective mother trees revealed absolute identity. Thus, mother trees could be identified by genotyping their fruits or seeds. The results demonstrate the applicability of DNA fingerprinting to woody and/or dry seed tissues without the destruction of embryos and endosperm or a significant contamination. Progress is now expected in dispersal biology as well as in forensics and forest management.