Ultrarapid engineering of biomimetic materials and tissues: Fabrication of nano- and microstructures by plastic compression
Ultrarapid engineering of biomimetic materials and tissues: Fabrication of nano- and microstructures by plastic compression
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DOI：
10.1002/adfm.200500042
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发表时间：
2005-11-01
影响因子：
19
通讯作者：
Nazhat, SN
中科院分区：
文献类型：
--
作者：
Brown, RA;Wiseman, M;Nazhat, SN
目前，工程组织的概念取决于培养细胞在支架周围制造新组织的能力。Ibis的速度本来就很慢，而且价格昂贵，迄今为止取得的成功有限。我们在这里报告了一个新的过程中的细胞无关的，控制工程的仿生支架，快速去除流体从过度水合胶原蛋白凝胶（或其他）结构，使用塑料压缩（PC）。PC制造产生致密的、细胞状的、机械强度强的天然胶原结构，具有可控的纳米和微米级仿生结构。大规模的收缩（> 100倍）提供了引入可控的机械性能、微分层和压花界面的能力。拓扑结构没有细胞参与，但具有高细胞活力。关键是，这需要几分钟而不是传统的几天和几周。PC制造过程的快速性和仿生潜力为生物材料和患者定制组织的生产开辟了新的途径。它也代表了“工程”组织的一个新概念。
Currently, the concept of engineered tissues depends on the ability of cultured cells to fabricate new tissue around a scaffold. Ibis is inherently slow and expensive and has had limited success so far. We report here a new process for the cell-independent, controlled engineering of biomimetic scaffolds by rapid removal of fluid from hyperhydrated collagen gel (or other) constructs, using plastic compression (PC). PC fabrication produces dense, cellular, mechanically strong native collagen structures with controllable nano- and microscale biomimetic structures. The huge-scale shrinkage (> 100-fold) provides the ability to introduce controllable mechanical properties, microlayering, and embossed interface. topography without cell participation, but with high cell viability. Critically, this takes minutes rather than the conventional days and weeks. The rapidity and biomimetic potential of the PC fabrication process at the mesoscale opens a new route for the production of biomaterials and patient-customized tissues. It also represents a new concept in 'engineering' tissues.