Bactericidal Effect of a Photoresponsive Carbon Monoxide-Releasing Nonwoven against Staphylococcus aureus Biofilms
Bactericidal Effect of a Photoresponsive Carbon Monoxide-Releasing Nonwoven against Staphylococcus aureus Biofilms
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DOI：
10.1128/aac.00703-16
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发表时间：
2016-07-01
影响因子：
4.9
通讯作者：
Schiller, Alexander
中科院分区：
文献类型：
--
作者：
Klinger-Strobel, Mareike;Glaeser, Steve;Schiller, Alexander
金黄色葡萄球菌是皮肤和皮肤结构感染的主要病原体，包括与生物膜形成相关的手术和创伤感染。由于生物膜的形成伴随着包埋细菌的高表型抗性，它们几乎不可能被常规抗生素根除。因此，替代的治疗策略是高度关注的。我们通过光响应一氧化碳（CO）释放分子[CORM-1，Mn-2（CO）（10）]和聚合物聚丙交酯的静电纺丝产生纳米结构的混合非织造物。该非织造布显示出CO诱导的抗微生物活性，其足以在405 nm下的光刺激后将生物膜嵌入的细菌减少70%。CORM释放的CO增加了生物膜中活性氧（ROS）的浓度，表明ROS除了抑制电子传递链外，还可能在CORM对S.金黄色。非织造布在较长时间暴露后显示出对真核细胞的细胞毒性增加，最可能是由于释放的乳酸，这对于局部和短时间治疗可能是可接受的。因此，CO释放无纺布可能是一种有前途的局部抗菌治疗生物膜相关的皮肤伤口感染。
Staphylococcus aureus is a leading pathogen in skin and skin structure infections, including surgical and traumatic infections that are associated with biofilm formation. Because biofilm formation is accompanied by high phenotypic resistance of the embedded bacteria, they are almost impossible to eradicate by conventional antibiotics. Therefore, alternative therapeutic strategies are of high interest. We generated nanostructured hybrid nonwovens via the electrospinning of a photoresponsive carbon monoxide (CO)-releasing molecule [CORM-1, Mn-2(CO)(10)] and the polymer polylactide. This nonwoven showed a CO-induced antimicrobial activity that was sufficient to reduce the biofilm-embedded bacteria by 70% after photostimulation at 405 nm. The released CO increased the concentration of reactive oxygen species (ROS) in the biofilms, suggesting that in addition to inhibiting the electron transport chain, ROS might play a role in the antimicrobial activity of CORMs on S. aureus. The nonwoven showed increased cytotoxicity on eukaryotic cells after longer exposure, most probably due to the released lactic acid, that might be acceptable for local and short-time treatments. Therefore, CO-releasing nonwovens might be a promising local antimicrobial therapy against biofilm-associated skin wound infections.