SPONTANEOUS SHEDDING OF PLASMA-MEMBRANE FRAGMENTS BY HUMAN CELLS INVIVO AND INVITRO
SPONTANEOUS SHEDDING OF PLASMA-MEMBRANE FRAGMENTS BY HUMAN CELLS INVIVO AND INVITRO
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DOI：
10.1016/0009-8981(77)90054-7
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发表时间：
1977-01-01
影响因子：
5
通讯作者：
ROELS, F
中科院分区：
文献类型：
--
作者：
DEBROE, ME;WIEME, RJ;ROELS, F
人十二指肠液、直肠绒毛状腺瘤分泌液、尿液和HeLa细胞培养基中含有质膜碎片，这些碎片可通过不同培养基中的电泳、Sepharose 4-B上的凝胶过滤、电子显微镜和细胞化学显示，它们携带与原始细胞膜相同比例的质膜酶（碱性磷酸酶EC 3.1.3.1、亮氨酸氨肽酶EC 3.4.1.1、5 '-核苷酸酶EC 3.1.3.5、麦芽糖酶EC 3.2.1.20）。平衡密度离心导致回收的这些质膜碎片的密度在CsCl中为1.190（g/ml），在蔗糖中为1.165（g/ml），在甲泛葡胺中为1.135（g/ml）。这些观察结果证明，整个质膜碎片脱落（koinozymic脱落）是活细胞的一个普遍特征。
Human duodenal fluid, secretion fluid of a villous adenoma of the rectum, urine and culture medium of HeLa cells contain plasma membrane fragments which can be revealed by electrophoresis in different media, gel filtration on Sepharose 4-B, electron microscopy and cytochemistry.They carry plasma membrane enzymes (alkaline phosphatase EC 3.1.3.1, leucine aminopeptidase EC 3.4.1.1, 5'-nucleotidase EC 3.1.3.5, maltase EC 3.2.1.20) in the same ratio as the membranes of the cells of origin. Equilibrium density centrifugation results in recovery of these plasma membrane fragments at density 1.190 (g/ml) in CsCl, 1.165 (g/ml) in sucrose, and 1.135 (g/ml) in metrizamide.Similar plasma membrane fragments were described previously in the serum of certain liver patients. These observations give evidence that shedding of whole plasma membrane fragments (koinozymic shedding) is a widespread feature of viable cells.