Antisense RhMLO1 Gene Transformation Enhances Resistance to the Powdery Mildew Pathogen in Rosa multiflora
Antisense RhMLO1 Gene Transformation Enhances Resistance to the Powdery Mildew Pathogen in Rosa multiflora
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DOI：
10.1007/s11105-015-0862-1
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发表时间：
2015-02
影响因子：
2.1
通讯作者：
Xianqin Qiu;Qi-gang Wang;Hao Zhang;H. Jian;Ningning Zhou;C. Ji;Huijun Yan;M. Bao;K. Tang
中科院分区：
文献类型：
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作者：
Xianqin Qiu;Qi-gang Wang;Hao Zhang;H. Jian;Ningning Zhou;C. Ji;Huijun Yan;M. Bao;K. Tang
白粉病是世界范围内最重要的作物病害之一。 遗传分析显示，Mlo基因的突变等位基因引起对在大肠杆菌中发现的病原体的广谱抗性。 麦片本研究探讨了利用转基因技术诱导植物对白粉病（Podosphaera pannosa）抗性的可能性， 玫瑰花的技术被研究了。转基因株系被证实为 基于PCR和Southern印迹分析转基因的整合和拷贝数。一 基于实时荧光光谱， 定量PCR和抗性分析。与对照植物相比， 转基因玫瑰表现出更高的抗性水平。此外，结果 表明RhMLO 1在 玫瑰-白粉病病原菌互作这表明了潜在的可行性 Mlohomologs的部分渲染策略 通过转基因方法或诱变使其无功能，以对抗粉状 蔷薇白粉病。据我们所知， 该研究是首次尝试使用基于aMlo的抗性策略来对抗Rosa中的白粉病。
Powdery mildew is one of the most important crop diseases worldwide. Genetic analysis has revealed that mutant alleles of theMlogene cause broad-spectrum resistance against pathogens found in cereal. In this study, the possibility of inducing powdery mildew (Podosphaera pannosa) resistance via transgenic technology in the rose was examined. The transgenic lines were confirmed for integration and copy number of the transgenes based on PCR and Southern blots. A clear correlation was found between resistance and the accumulation of silencedRhMLO1, based on real-time fluorescent quantitative PCR and resistance analysis. Compared with the control plants, transgenic roses displayed higher resistance levels. Furthermore, the results indicated thatRhMLO1has a negative role in the rose–powdery mildew pathogen interaction. This demonstrates the potentially viable strategy of rendering theMlohomologs partially non-functional via a transgenic approach or mutagenesis in order to counter powdery mildew inRosa. To the best of our knowledge, this study is one of the first attempts at using aMlo-based resistance strategy to combat powdery mildew inRosa.