Temperature dependence of ATP hydrolysis and calcium uptake by fragmented sarcoplasmic membranes.
Temperature dependence of ATP hydrolysis and calcium uptake by fragmented sarcoplasmic membranes.
复制标题
ATP 水解和碎片肌质膜钙吸收的温度依赖性。
DOI：
10.1016/0003-9861(67)90051-3
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发表时间：
1967
影响因子：
3.9
通讯作者：
S. Watanabe
中科院分区：
文献类型：
--
作者：
G. Inesi;S. Watanabe
从兔骨骼肌制备的肌质膜碎片的钙摄取和ATP水解的初始速率也发现了类似的温度依赖性。草酸盐的加入改变了反应混合物的钙吸收和ATP分裂的温度依赖性。非钙依赖性（“基础”）ATP水解显示出与钙依赖性（“额外”）ATP分裂不同的温度依赖性。间接证据表明，相当数量的钙与膜结合（40-50 μ mol/gm蛋白质）。该量构成在不存在草酸盐的情况下吸收的总钙的显著部分，但与在草酸盐存在下在囊泡内运输的更大量相比，其可忽略不计。
A similar temperature dependence has been found for the initial rates of calcium uptake and ATP hydrolysis by fragmented sarcoplasmic membranes prepared from rabbit skeletal muscle. The addition of oxalate to the reaction mixture changed the temperature dependence of both calcium uptake and ATP splitting. Calcium-independent (“basal”) ATP hydrolysis displayed a temperature dependence different from that of calcium-dependent (“extra”) ATP splitting. Indirect evidence is shown to indicate that a considerable amount of calcium is bound to the membrane (40–50 μmoles/gm protein). This amount constitutes a significant portion of the total calcium taken up in the absence of oxalate, but it is negligible in comparison to the larger amount transported inside the vesicles in the presence of oxalate.