Saturated molecular map of the rice genome based on an interspecific backcross population.
Saturated molecular map of the rice genome based on an interspecific backcross population.
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DOI：
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发表时间：
1994-12
期刊：
影响因子：
3.3
通讯作者：
M. Causse;Theresa;-M.;Fulton;Yong-Gu Cho;Sang;J. Chunwongse;Jinhua Xiao;Sandra;-E.;Hanington;G. Sécond;S. McCouch;S. Tanksley
中科院分区：
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作者：
M. Causse;Theresa;-M.;Fulton;Yong-Gu Cho;Sang;J. Chunwongse;Jinhua Xiao;Sandra;-E.;Hanington;G. Sécond;S. McCouch;S. Tanksley
构建了由726个标记(主要是限制性片段长度多态；RFLP)组成的水稻基因组分子图谱。作图群体是栽培水稻与其非洲野生近缘长秆野生稻之间的回交。这些物种之间的高度多态，再加上聚合酶链式反应扩增的cDNA文库的使用，有助于提高作图效率。本研究中使用的探针的一个子集以前被用来构建来自亚种间杂交的RFLP图谱，为比较两个图谱以及两个杂交的相对作图效率提供了基础。除上述PstI基因组文库外，还利用了来自水稻(Oryza)、燕麦(Avena)和大麦(Hordeum)的3个cDNA文库。讨论了每种方法检测到的多态水平和识别用于水稻作图的异源序列的频率。尽管繁殖障碍很强，但在这个回交群体中表现出扭曲分离的标记百分比与以前用于定位的种内F2群体没有显著不同。地图全长1491 cm，框架图平均间距为4.0 cm，总体长度为2.0 cm。利用PstI水稻基因组文库中的238个标记、水稻(Oryza)文库中的250个标记、燕麦(Avena)中的112个标记、大麦(Hordeum)文库中的20个标记、2个玉米基因组克隆(ZEA)、11个微卫星标记、3个端粒标记、11个同工酶、26个克隆基因、6个随机扩增多态性DNA标记和47个突变表型进行了定位。讨论了分子图谱在植物改良中的应用。
A molecular map has been constructed for the rice genome comprised of 726 markers (mainly restriction fragment length polymorphisms; RFLPs). The mapping population was derived from a backcross between cultivated rice, Oryza sativa, and its wild African relative, Oryza longistaminata. The very high level of polymorphism between these species, combined with the use of polymerase chain reaction-amplified cDNA libraries, contributed to mapping efficiency. A subset of the probes used in this study was previously used to construct an RFLP map derived from an inter subspecific cross, providing a basis for comparison of the two maps and of the relative mapping efficiencies in the two crosses. In addition to the previously described PstI genomic rice library, three cDNA libraries from rice (Oryza), oat (Avena) and barley (Hordeum) were used in this mapping project. Levels of polymorphism detected by each and the frequency of identifying heterologous sequences for use in rice mapping are discussed. Though strong reproductive barriers isolate O. sativa from O. longistaminata, the percentage of markers showing distorted segregation in this backcross population was not significantly different than that observed in an intraspecific F2 population previously used for mapping. The map contains 1491 cM with an average interval size of 4.0 cM on the framework map, and 2.0 cM overall. A total of 238 markers from the previously described PstI genomic rice library, 250 markers from a cDNA library of rice (Oryza), 112 cDNA markers from oat (Avena), and 20 cDNA markers from a barley (Hordeum) library, two genomic clones from maize (Zea), 11 microsatellite markers, three telomere markers, eleven isozymes, 26 cloned genes, six RAPD, and 47 mutant phenotypes were used in this mapping project. Applications of a molecular map for plant improvement are discussed.