Metabolism of arsenic after acute occupational arsine intoxication
Metabolism of arsenic after acute occupational arsine intoxication
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DOI：
10.1080/009841097159610
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发表时间：
1997-11-01
期刊：
影响因子：
--
通讯作者：
Leone, R
中科院分区：
文献类型：
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作者：
Apostoli, P;Alessio, L;Leone, R
在与毒理学相关的元素中，无机砷(As)可能表现出最复杂的代谢，我们认为鉴定和定量职业性急性砷中毒后排出的不同As形态是有趣的。为此，采用液相色谱与电感耦合等离子体质谱联用的混合分析方法测定了总Ar和5种As的含量。尿总砷排泄量以入院后5d最高。As的主要排泄物为单甲基砷酸盐(MMA)、二甲基砷酸盐(DMA)、Ar3+砷甜菜碱(ASB)，其次为5+。受试者通过食物摄入ASB似乎并不能完全证明受试者尿中ASB排泄量的合理性。砷主要通过尿液排泄，清除量为7.8ml/h/kg，遵循三相模型，周期分别为28h、59h和9d。DMA排泄在几天后达到顶峰，此时无机形式的排泄大幅减少(而MMA的排泄仍在增加)，这似乎证实了存在两个连续的甲基化酶活性。此外，As从血液中的消除速率遵循三相模型，不同物种的半衰期从27到86小时不等，梯度如下：5+<；MMA<；As3+<；DMA<；ASB。
Among the elements of toxicological relevance, inorganic arsenic (As) probably exhibits the most complex metabolism, and we deemed it interesting to identify and quantify the different As species excreted after an occupational acute intoxication with arsine. For this purpose total Ar and five As species were determined using an hybrid analytical method coupling liquid chromatography with inductively coupled plasma mass spectrometry. The highest urinary elimination of total As was observed in the first 5 d after admission. The As species mostly excreted were monomethylarsonate (MMA), dimethylarsinate (DMA), Ar3+ arsenobetaine (AsB), and to a lesser extent As5+. The amount of AsB excreted in urine by the subject does not appear to be completely justified by AsB intake through food. Arsenic is excreted mainly via the urine with a clearance of 7.8 ml/h/kg and follows a triphasic model with periods of 28 h, 59 h, and 9 d, respectively. The evidence that DMA excretion culminates after a few days, when the excretion of the inorganic form is substantially reduced (while that of MMA is still elevated), seems to confirm the existence of two successive methylating enzyme activities. Furthermore, the elimination rate of As from blood follows a three-phase model and the half-lives of different species vary from about 27 to 86 h with the following gradient As5+ < MMA < As3+ < DMA < AsB.