The effect of hydrogel charge density on cell attachment
The effect of hydrogel charge density on cell attachment
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DOI：
10.1016/j.biomaterials.2003.09.084
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发表时间：
2004-07-01
期刊：
影响因子：
14
通讯作者：
Keller, J
中科院分区：
文献类型：
--
作者：
Schneider, GB;English, A;Keller, J
成骨细胞和成纤维细胞的竞争性生长模式可以决定在伤口部位是否形成健康的骨或病理性瘢痕组织。细胞与用于组织工程应用的各种异体生物材料的相互作用是复杂的。确定的合成培养基对于研究离子和细胞受体特异性相互作用是有价值的。本研究的目的是确定是否成纤维细胞和成骨细胞差异连接到HEMA和PEG水凝胶共聚与正，负，或中性电荷密度，或当接枝有特定的整合素受体RGD粘附配体。细胞骨架表型进行了评估，免疫荧光显微镜和细胞附着试验。与成纤维细胞相比，成骨细胞对HEMA和PEG水凝胶的附着显著更高（P < 0.01）。带正电的HEMA和PEG水凝胶支持最大的细胞附着，其次是RGD接枝，负，中性电荷密度，分别。与成纤维细胞相比，这些条件中的每一种都引起成骨细胞附着增加近两倍。将细胞附着于血清包被的盖玻片用作对照。免疫荧光分析表明，这两种细胞类型的附着和扩散更好地带正电荷的水凝胶。然而，与成骨细胞相比，成纤维细胞表现出较少的扩散。总之，不同的亲水性差异影响成骨细胞和成纤维细胞的附着和扩散。(C)2003爱思唯尔有限公司。保留所有权利。
The competitive growth patterns of osteoblasts and fibroblasts can determine if healthy bone or pathologic scar tissue is formed at a wound site. Cell interactions with various alloplastic biomaterials used for tissue-engineering applications is complex. Defined synthetic mediums are valuable for studying ionic and cell receptor specific interactions. The objectives of this study were to determine if fibroblasts and osteoblasts differentially attached to HEMA and PEG hydrogels copolymerized with positive, negative, or neutral charge densities, or when grafted with specific integrin receptor RGD adhesion ligand. Cytoskeletal phenotypes were assessed with immunofluorescent microscopy and cell attachment assays. Osteoblast cell attachment to both HEMA and PEG hydrogels was significantly higher (P < 0.01) as compared to fibroblast cells. Positively charged HEMA and PEG hydrogels supported the greatest cell attachment, followed by RGD grafted, negative, and neutral charge densities, respectively. Each of these conditions elicited nearly a two-fold increase in osteoblast cell attachment, as compared to fibroblasts. Cell attachment to serum-coated coverslips was used as the control. Immunofluorescent analysis showed that both cell types attached and spread better on the positively charged hydrogels. However, fibroblasts demonstrated less spreading as compared to osteoblasts. In conclusion, differences in hydrophilic properties differentially affect osteoblast and fibroblast cell attachment and spreading. (C) 2003 Elsevier Ltd. All rights reserved.