PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF INTERACTIONS BETWEEN ISOLATES OF PERONOSPORA-PARASITICA AND ACCESSIONS OF ARABIDOPSIS-THALIANA
PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF INTERACTIONS BETWEEN ISOLATES OF PERONOSPORA-PARASITICA AND ACCESSIONS OF ARABIDOPSIS-THALIANA
复制标题
DOI：
10.1094/mpmi-7-0223
复制
发表时间：
1994-03-01
影响因子：
3.5
通讯作者：
CRUTE, IR
中科院分区：
文献类型：
--
作者：
HOLUB, EB;BEYNON, LJ;CRUTE, IR
野生十字花科植物拟南芥（Arabidopsis thaliana）与霜霉病菌（Peronospora parasitica）之间的寄生共生关系为探索植物防御的进化和分子基础提供了极好的机会。通过描述在这种共生关系中观察到的表型和基因型变异，为研究基因型特异性识别过程奠定了基础。互作表型，结合方面的主机响应和寄生虫的发展，本文描述了11个主机加入和7个寄生虫分离物的组合。寄生虫无性繁殖的时间和程度在组合中各不相同，并提供了一个间接的评估殖民化。宿主细胞的坏死范围从接种后7天肉眼可见的微小绒毛到3天清晰可见的更广泛的凹陷，并经常扩大，直到整个子叶坏死。12个与不同互作表型相关的宿主基因座被部分假定为。研究了9个A.包括重组近交系的高世代用于确认基因座的身份。两个宿主种质之间的单次杂交可用于表征和定位与寄生疫霉的分离物特异性识别和不同的相互作用表型相关的几个基因座。部分显性和遗传上位性被假定为这种寄生共生的共同特征。
The parasitic symbiosis between the wild crucifer Arabidopsis thaliana and the biotrophic oomycete Peronospora parasitica (downy mildew) provides an excellent opportunity to explore the evolution and molecular basis of plant defense. By describing the phenotypic and genotypic variation observed in this symbiosis, a foundation has been laid for investigating the process of genotype-specific recognition. Interaction phenotypes, incorporating aspects of both host response and parasite development, are described in this paper for combinations of 11 host accessions and seven parasite isolates. The timing and degree of asexual reproduction by the parasite varied among the combinations and provided an indirect assessment of colonization. Necrosis of host cells ranged from minute flecks visible macroscopically 7 days after inoculation (dai) to more extensive pits clearly visible 3 dai and often expanding until the entire cotyledon was necrotic 7 dai. Twelve host loci associated with different interaction phenotypes were postulated in part by. studying segregation among F-2 progeny from crosses of a half-diallel among nine A. thaliana accessions; advanced generations including recombinant inbred lines were used to confirm the identity of loci. A single cross between two host accessions can be used to characterize and map several loci associated with isolate-specific recognition of P. parasitica and distinct interaction phenotypes. Partial dominance and genetic epistasis are postulated as being common features of this parasitic symbiosis.