Cross-Species Comparison of the Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei Quorum-Sensing Regulons
Cross-Species Comparison of the Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei Quorum-Sensing Regulons
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DOI：
10.1128/jb.01974-14
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发表时间：
2014-11-01
影响因子：
3.2
通讯作者：
Greenberg, E. Peter
中科院分区：
文献类型：
--
作者：
Majerczyk, Charlotte D.;Brittnacher, Mitchell J.;Greenberg, E. Peter
类鼻疽伯克霍尔德菌、泰国伯克霍尔德菌和鼻疽伯克霍尔德菌（Bptm组）是近亲，但生活方式截然不同：B。类鼻疽是一种条件致病菌，B。thailandensis是一种非致病菌，B.鼻疽是一种受宿主限制的病原体。这三个物种的酰基高丝氨酸内酯群体感应（QS）系统显示出高度的保守性。我们使用转录组测序（RNA-seq）来定义每个物种中的群体感应调节子，并对QS控制的直系同源物进行了跨物种分析。我们的分析揭示了一组核心的QS调节基因在所有三个物种，以及QS控制的因素，只有两个物种共享或独特的给定物种。这项对B的QS调节子的全球调查。类鼻疽B. B. thailandensis和B.鼻疽作为一个平台，预测QS控制的过程可能是重要的，在不同的细菌生态位，并有助于B的发病机制。类鼻疽和B.鼻疽
Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei (the Bptm group) are close relatives with very different lifestyles: B. pseudomallei is an opportunistic pathogen, B. thailandensis is a nonpathogenic saprophyte, and B. mallei is a host-restricted pathogen. The acyl-homoserine lactone quorum-sensing (QS) systems of these three species show a high level of conservation. We used transcriptome sequencing (RNA-seq) to define the quorum-sensing regulon in each species, and we performed a cross-species analysis of the QS-controlled orthologs. Our analysis revealed a core set of QS-regulated genes in all three species, as well as QS-controlled factors shared by only two species or unique to a given species. This global survey of the QS regulons of B. pseudomallei, B. thailandensis, and B. mallei serves as a platform for predicting which QS-controlled processes might be important in different bacterial niches and contribute to the pathogenesis of B. pseudomallei and B. mallei.