Engineering a gold-specific regulon for cell-based visual detection and recovery of gold
Engineering a gold-specific regulon for cell-based visual detection and recovery of gold
复制标题
设计金特异性调节子，用于基于细胞的金视觉检测和回收
DOI：
10.1039/c2sc01119k
复制
发表时间：
2012-01-01
期刊：
影响因子：
8.4
通讯作者：
Zhao, Jing
中科院分区：
文献类型：
--
作者：
Wei, Wei;Zhu, Tianze;Zhao, Jing
将来自沙门氏菌GOL调节子的金特异感觉蛋白GOLS与下游的红色荧光蛋白相结合，实现了肉眼高灵敏和选择性的全细胞检测金离子。GOL调节子中假定的黄金伴侣Golb随后被证实是金(I)离子的特异性，而不是包括铜(I)在内的其他金属离子。随后在大肠杆菌细胞表面展示的Golb允许从含有各种亲硫金属离子的介质中选择性地富集金离子。进一步证明，细胞表面富集金(I)很容易回收，缺金细菌能够重复使用。含有来自GOL调节子的这些金特异元件的大肠杆菌可能是一种有价值的工具，用于从环境资源中直观地检测和方便地回收金离子。
A gold-specific sensory protein GolS from Samonella gol regulon was incorporated into E. coli, which in conjunction with an engineered downstream red fluorescence protein allowed the highly sensitive and selective whole-cell detection of gold(III) ions by naked eyes. The putative gold-chaperone, GolB, in the gol regulon was next verified to be specific to gold(I) ions over other metal ions including copper(I). The subsequent display of GolB on E. coli cell surface permitted selective enrichment of gold ions from media containing various thiophilic metal ions. The cell surface-enriched gold(I) was further shown to be easily recovered and the gold-deprived bacteria were capable for re-usage. E. coli bacteria harboring these gold-specific elements from the gol regulon could be a valuable tool for visual detection and facile recycling of gold ions from environmental resources.