Frontline:: A B220+ CD117+ CD19- hematopoietic progenitor with potent lymphoid and myeloid developmental potential
Frontline:: A B220+ CD117+ CD19- hematopoietic progenitor with potent lymphoid and myeloid developmental potential
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DOI：
10.1002/eji.200526318
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发表时间：
2005-07-01
影响因子：
5.4
通讯作者：
Rolink, AG
中科院分区：
文献类型：
--
作者：
Balciunaite, G;Ceredig, R;Rolink, AG
在这份报告中，我们在正常小鼠的骨髓（BM）中鉴定了具有有效淋巴和骨髓发育潜能的B220（+）CD 117（+）CD 19（-）NK1.1（-）细胞亚群。这些细胞占有核BM细胞的0.1-0.2%。通过在基质细胞和细胞因子的适当组合存在下的有限稀释分析，1/5-10的分选细胞形成B细胞，1/10-15形成T细胞，1/5-10产生巨噬细胞。当在表达Notch配体Delta-like-1的OP 9基质和OP 9基质细胞的混合物上培养时，单个细胞产生T和B细胞。静脉输注后，新鲜分选的细胞瞬时重建T和B细胞祖细胞区室，产生成熟T和B淋巴细胞群。讨论了野生型小鼠B220+ CD 117 + CD 19- NK1.1-细胞与其他多系骨髓祖细胞的关系。
In this report, we identify in the bone marrow (BM) of normal mice a subpopulation of B220(+) CD117(+) CD19(-) NK1.1(-) cells with potent lymphoid and myeloid developmental potential. These cells represent 0.1-0.2% of nucleated BM cells. By limiting dilution analysis in the presence of the appropriate combination of stromal cells and cytokines, 1 in 5-10 sorted cells formed B cells, 1 in 10-15 formed T cells and 1 in 5-10 generated macrophages. When cultured on a mixture of OP9 stroma and OP9 stromal cells expressing the Notch ligand Delta-like-1, single cells generated both T and B cells. Following intravenous infusion, freshly sorted cells transiently reconstituted both the T and B cell progenitor compartments, generating cohorts of mature T and B lymphocytes. The relationship between B220+ CD117+ CD19- NK1.1- cells of wild-type mice and other multi-lineage BM progenitors is discussed.