Red cell N5-methyltetrahydrofolate concentrations and C677T methylenetetrahydrofolate reductase genotype in patients with stroke
Red cell N5-methyltetrahydrofolate concentrations and C677T methylenetetrahydrofolate reductase genotype in patients with stroke
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脑卒中患者红细胞 N5-甲基四氢叶酸浓度和 C677T 亚甲基四氢叶酸还原酶基因型
DOI：
10.1136/jcp.57.1.54
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发表时间：
2003
影响因子：
3.4
通讯作者：
J. Eikelboom
中科院分区：
文献类型：
--
作者：
G. Icke;M. Dennis;S. Sjollema;D. Nicol;J. Eikelboom
目的：探讨卒中患者红细胞总叶酸、红细胞N5-甲基四氢叶酸(N5MTHF)浓度与N5N10-亚甲基四氢叶酸还原酶(MTHFR)基因分型的关系。方法：这项研究包括120名连续入院的急性中风患者。记录了复合维生素补充剂的使用情况。用干酪乳杆菌和粪肠球菌进行微生物测定，用DPC-BioMediq IMMULITE™2000型分析仪测定血清和红细胞叶酸。测定血浆总同型半胱氨酸(THcy)、血清钴胺、血清维生素B6，并测定C677T亚甲基四氢叶酸还原酶(MTHFR)基因。结果：在整个患者队列中，根据MTHFR基因的不同，血tHcy或维生素浓度没有显著差异。然而，排除服用维生素的患者后，TT基因携带者的红细胞总叶酸和红细胞N5MTHF显著低于CT或CC基因携带者。在整个队列中，无论基因型如何，微生物学检测的红细胞叶酸显著低于IMMULITE检测。在排除服用维生素的患者后，这种差异仍然存在。结论：与CT和TT MTHFR基因型相比，伴有TT的卒中患者红细胞总叶酸和红细胞N5MTHF显著降低，尤其是不服用维生素补充剂的患者。与IMMULITE™分析相比，测量生物活性叶酸的微生物分析提供的叶酸估计值要低得多。由于叶酸是血液同型半胱氨酸值的关键决定因素，这些发现可能会影响对大多数流行病学研究中报道的同型半胱氨酸升高与动脉粥样硬化血栓风险之间联系的强度和独立性的解释。
Aims: To investigate the relation between total red cell folate, red cell N5-methyltetrahydrofolate (N5MTHF) concentrations, and N5N10-methylenetetrahydrofolate reductase (MTHFR) genotypes in stroke. Methods: The study comprised 120 consecutive patients presenting to hospital with acute stroke. Multivitamin supplement use was recorded. Serum and red cell folate were measured by microbiological assays using Lactobacillus casei and Enterococcus faecalis, and by the DPC-BioMediq Immulite™ 2000 analyser. Total plasma homocysteine (tHcy), serum cobalamin, and serum vitamin B6 were measured and the C677T MTHFR genotype determined. Results: There were no significant differences in blood tHcy or vitamin concentrations according to MTHFR genotype in the overall patient cohort. However, when patients taking vitamins were excluded, total red cell folate and red cell N5MTHF were significantly lower in patients with the TT genotype compared with CT or CC genotypes. In the overall cohort, irrespective of genotype, red cell folate was significantly lower when assayed microbiologically than with the Immulite assay. This discrepancy remained after exclusion of patients taking vitamins. Conclusion: Total red cell folate and red cell N5MTHF are significantly lower in stroke patients with the TT compared with the CT and TT MTHFR genotypes, particularly those not taking vitamin supplements. Microbiological assays that measure biologically active folates provide substantially lower estimates of folate than the Immulite™ assay. Because folate is a key determinant of blood homocysteine values, these findings may impact on the interpretation of the strength and independence of the association between raised blood concentrations of homocysteine and atherothrombosis risk reported in most epidemiological studies.
影响因子：
37.8
作者：
Jacques, PF;Bostom, AG;Rozen, R
通讯作者：
Rozen, R