SNARE-mediated retrograde traffic from the Golgi complex to the endoplasmic reticulum
SNARE-mediated retrograde traffic from the Golgi complex to the endoplasmic reticulum
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DOI：
10.1016/s0092-8674(00)81097-1
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发表时间：
1996-04-19
期刊：
影响因子：
64.5
通讯作者：
Pelham, HRB
中科院分区：
文献类型：
--
作者：
Lewis, MJ;Pelham, HRB
真核细胞分泌途径的运作需要运输囊泡与合适的靶细胞器的选择性对接和融合。这部分是由称为v-SNARE（在囊泡上）和t-SNARE（在靶膜上）的整合膜蛋白介导的。我们描述了一种新的酵母t-SNARE，驻留在内质网和介导从高尔基复合体逆行交通。该蛋白的突变阻止HDEL受体和携带二元回收信号的膜蛋白再循环到内质网。前向流量也被阻塞，但只是间接的。与其他酵母突变体的比较表明，Sec 21 p（γ-COP）和Sec 20 p（内质网膜蛋白）也主要参与，如果不是唯一的，在逆行运输。
Operation of the secretory pathway in eukaryotic cells requires the selective docking and fusion of transport vesicles with the appropriate target organelle. This is mediated in part by integral membrane proteins termed v-SNAREs (on vesicles) and t-SNAREs (on the target membranes). We describe a novel yeast t-SNARE that resides on the endoplasmic reticulum and mediates retrograde traffic from the Golgi complex. Mutation of this protein prevents both the HDEL receptor and a membrane protein bearing a dibasic retrieval signal from recycling to the endoplasmic reticulum. Forward traffic is also blocked, but only indirectly. Comparison with other yeast mutants indicates that Sec21p (gamma-COP) and Sec20p (an endoplasmic reticulum membrane protein) are also involved primarily, if not exclusively, in retrograde transport.