Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis
Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis
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DOI：
10.1104/pp.106.094185
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发表时间：
2007-03-01
期刊：
影响因子：
7.4
通讯作者：
Hsieh, Hsu-Liang
中科院分区：
文献类型：
--
作者：
Chen, Ing-Chien;Huang, I-Ching;Hsieh, Hsu-Liang
远红不敏感219（FIN 219）先前被证明参与光敏色素A介导的FR光信号传导。为了进一步了解其功能和与其他光信号组分的调控关系，使用酵母双杂交方法分离FIN 219相互作用的伴侣。在这里，我们证明了FIN 219相互作用蛋白1（FIP 1）在体外和体内与FIN 219相互作用，由217个氨基酸组成，属于大谷胱甘肽S-转移酶基因家族的tau类。FIP 1进一步显示具有谷胱甘肽S-转移酶活性。FIP 1功能的获得和部分丧失导致下胚轴在连续FR（cFR）光下表现为低敏感表型，在长日照条件下表现为延迟开花表型，这表明FIP 1可能以复合物的形式存在，在拟南芥（Arabidopsis thaliana）发育的调控中发挥作用。此外，FIP 1 mRNA在光敏色素A-105 1突变体的抑制子中表达下调，在cFR条件下，FIP 1 mRNA在组成型光形态发生突变体cop 1 - 4和cop 1 -5中表达差异。有趣的是，FIP 1的表达上调fin 219突变体在所有的光条件下，除了cFR。此外，启动子活性测定显示，FIP 1的表达是光依赖性的，主要与血管组织，和发育调控。亚细胞定位研究表明，β-葡萄糖醛酸酶-FIP 1融合蛋白定位于细胞核和细胞质。综上所述，这些数据表明FIP 1可能与FIN 219相互作用，以调节细胞响应光的伸长和开花。
Far-red (FR) insensitive 219 (FIN219) was previously shown to be involved in phytochrome A-mediated FR light signaling. To further understand its function and regulatory relation with other light-signaling components, a yeast two-hybrid approach was used to isolate FIN219-interacting partners. Here, we demonstrate that FIN219-interacting protein 1 (FIP1) interacts with FIN219 in vitro and in vivo and is composed of 217 amino acids that belong to the tau class of the large glutathione S-transferase gene family. FIP1 was further shown to have glutathione S-transferase activity. The gain of function and partial loss of function of FIP1 resulted in a hyposensitive hypocotyl phenotype under continuous FR (cFR) light and a delayed flowering phenotype under long-day conditions, which suggests that FIP1 may exist in a complex to function in the regulation of Arabidopsis (Arabidopsis thaliana) development. In addition, FIP1 mRNA was down-regulated in the suppressor of phytochrome A-105 1 mutant and differentially expressed in constitutive photomorphogenic 1-4 (cop1-4) and cop1-5 mutants under cFR. Intriguingly, FIP1 expression was up-regulated in the fin219 mutant under all light conditions, except cFR. Furthermore, promoter activity assays revealed that FIP1 expression was light dependent, mainly associated with vascular tissues, and developmentally regulated. Subcellular localization studies revealed that the beta-glucuronidase-FIP1 fusion protein was localized in the nucleus and cytoplasm. Taken together, these data indicate that FIP1 may interact with FIN219 to regulate cell elongation and flowering in response to light.