Adrenocorticotrophic hormone modulates Escherichia coli O157:H7 adherence to porcine colonic mucosa.
Adrenocorticotrophic hormone modulates Escherichia coli O157:H7 adherence to porcine colonic mucosa.
复制标题
促肾上腺皮质激素调节大肠杆菌 O157:H7 对猪结肠粘膜的粘附。
DOI：
10.1080/10253890500188732
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Brown,DavidR
中科院分区：
文献类型：
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作者：
Schreiber,KristinL;Brown,DavidR
暴露于压力与疾病易感性相关，据报道，一种压力介质去甲肾上腺素可增强肠出血性大肠杆菌 O157:H7 (EHEC) 对结肠粘膜的粘附。我们测试了促肾上腺皮质激素（ACTH）和其他与压力相关的激素可能以类似方式发挥作用的假设。将幼猪的远端结肠粘膜外植体安装在 Ussing 室中，并将其管腔部分暴露于肠出血性大肠杆菌菌株 700728 中 30-90 分钟。当添加到腔内而非腔内沐浴介质中时，ACTH 在 90 分钟内以浓度依赖性方式增加 EHEC 粘附性 (EC50= 1.2 nM)，但不会改变组织电导。 ACTH 对猪适应的非 O157E 的粘附没有影响。粘菌素。在用 2 型黑皮质素受体拮抗剂 ACTH7-38、神经传导阻滞剂石房蛤毒素或毒蕈碱胆碱能拮抗剂阿托品对管腔预处理的组织中，可防止 0.1 μM ACTH 对管腔 EHEC 粘附的影响。此外，在没有 ACTH 的情况下，ACTH7-38 会降低 EHEC 的依从性。这些结果表明，ACTH 通过位于肠神经上的黑皮质素受体发挥作用，增强肠出血性大肠杆菌的粘膜粘附。
Exposure to stress is associated with susceptibility to disease and one stress mediator, norepinephrine, has been reported to enhance the adherence of enterohemorrhagicEscherichia coliO157:H7 (EHEC) to the colonic mucosa. We tested the hypothesis that adrenocorticotropic hormone (ACTH) and other stress-related hormones may act in a similar fashion. Explants of distal colonic mucosa from young pigs were mounted in Ussing chambers and their luminal aspect was exposed to EHEC strain 700728 for 30–90 min. When added to the contraluminal, but not luminal bathing medium, ACTH increased EHEC adherence within 90 min in a concentration-dependent manner (EC50= 1.2 nM), but did not alter tissue electrical conductance. ACTH had no effect on the adherence of a pig-adapted non-O157E. colistrain. The effect of 0.1 μM ACTH on luminal EHEC adherence was prevented in tissues pretreated contraluminally with the type 2 melanocortin receptor antagonist ACTH7–38, the neuronal conduction blocker saxitoxin, or the muscarinic cholinergic antagonist atropine. Moreover, ACTH7–38decreased EHEC adherence in the absence of ACTH. These results suggest that ACTH acts via melanocortin receptors located on enteric nerves to enhance mucosal adherence of EHEC.