Pathways for the anaerobic microbial debromination of polybrominated diphenyl ethers
Pathways for the anaerobic microbial debromination of polybrominated diphenyl ethers
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DOI：
10.1021/es0720917
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发表时间：
2008-04-15
影响因子：
11.4
通讯作者：
Alvarez-Cohen, Lisa
中科院分区：
文献类型：
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作者：
Robrock, Kristin R.;Korytar, Peter;Alvarez-Cohen, Lisa
采用全二维气相色谱法（GC x GC）研究了7种多溴联苯醚（PBDEs）在3种不同厌氧脱卤细菌培养物中的脱溴途径：分析的同系物是工业上使用的八溴二苯醚混合物的五种主要成分（八溴二苯醚196、203和197，七溴二苯醚183和六溴二苯醚153）以及环境中最常检测到的两种多溴二苯醚，五溴二苯醚99和四溴二苯醚47。在本研究中评价的脱卤培养物中，有一种富含三氯乙烯的聚生体，含有多种Dehalococcoides物种，还有两种纯培养物，限制性脱卤菌PER-K23和Reynniense脱硫杆菌PCP-1。多溴联苯醚样品采用气相色谱-气相色谱联用电子捕获检测器进行分析，以最大限度地分离和鉴定产品同系物。所有研究的同系物在一定程度上脱溴的三种文化，都表现出类似的脱溴途径，优先去除帕拉和Meta溴。高度溴化的同系物的脱溴过程极其缓慢，通常在三个月后转化的多溴二苯醚不到10%的nM浓度。相比之下，溴化程度较低的同系物（如penta 99和tetra 47）的脱溴速度更快，一些培养物在数周内就完全将nM水平的tetra 47脱溴。
The debromination pathways of seven polybrominated diphenyl ethers (PBDEs) by three different cultures of anaerobic dehalogenating bacteria were investigated using comprehensive two-dimensional gas chromatography (GC x GC): The congeners analyzed were the five major components of the industrially used octa-BDE mixture (octa-BDEs 196, 203, and 197, hepta-BDE 183, and hexa-BDE 153) as well as the two most commonly detected PBDEs in the environment, penta-BDE 99 and tetra-BDE 47. Among the dehalogenating cultures evaluated in this study were a trichloroethene-enriched consortium containing multiple Dehalococcoides species, and two pure cultures, Dehalobacter restrictus PER-K23 and Desulfitobacterium hafniense PCP-1. PBDE samples were analyzed by GC x GC coupled to an electron capture detector to maximize separation and identification of the product congeners. All studied congeners were debrominated to some extent by the three cultures and all exhibited similar debromination pathways with preferential removal of para and meta bromines. Debromination of the highly brominated congeners was extremely slow, with usually less than 10% of nM concentrations of PBDEs transformed after three months. In contrast, debromination of the lesser brominated congeners, such as penta 99 and tetra 47, was faster, with some cultures completely debrominating nM levels of tetra 47 within weeks.