The effect of methanol on citric acid production from galactose by Aspergillus niger
The effect of methanol on citric acid production from galactose by Aspergillus niger
复制标题
甲醇对黑曲霉利用半乳糖生产柠檬酸的影响
DOI：
10.1007/bf00261914
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发表时间：
2004
影响因子：
5
通讯作者：
John D. Brooks
中科院分区：
文献类型：
--
作者：
I. Maddox;M. Hossain;John D. Brooks
研究了甲醇对几种曲霉菌由半乳糖生产柠檬酸能力的影响。在没有甲醇的情况下，观察到很少的产量（小于1克/升）。然而，在甲醇存在的情况下（终浓度为1% v/v），柠檬酸的产量和产率显著提高。在无细胞提取物中，柠檬酸的产生与2-氧戊二酸脱氢酶和丙酮酸羧化酶的活性密切相关。在柠檬酸生产过程中，甲醇存在下，2-氧戊二酸脱氢酶活性低，丙酮酸羧化酶活性高。在没有甲醇的情况下，产生的柠檬酸很少，反之亦然。这表明，甲醇的存在可能会增加细胞对柠檬酸盐的渗透性，并且细胞通过抑制2-氧戊二酸脱氢酶来增加细胞内水平的降低。
The effect of methanol on the ability of several strains ofAspergillusto produce citric acid from galactose has been investigated. In the absence of methanol, very little production (less than 1 g/l) was observed. In the presence of methanol (final concentration 1% v/v), however, citric acid production and yeilds were increased considerably. Strong relationships were observed between citric acid production and the activities of the enzymes 2-oxoglutarate dehydrogenase and pyruvate carboxylase in cell-free extracts. During citric acid production, in the presence of methanol, the activity of 2-oxoglutarate dehydrogenase was low and that of pyruvate carboxylase high. In the absence of methanol, where little citric acid was produced, the reverse was true. It is suggested that the presence of methanol may increase the permeability of the cell to citrate, and the cell responds to the diminished intracellular level by increasing production via repression of 2-oxoglutarate dehydrogenase.