Functional beta-cell maturation is marked by an increased glucose threshold and by expression of urocortin 3.
Functional beta-cell maturation is marked by an increased glucose threshold and by expression of urocortin 3.
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DOI：
10.1038/nbt.2141
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发表时间：
2012-02-26
影响因子：
46.9
通讯作者：
Melton DA
中科院分区：
文献类型：
--
作者：
Blum B;Hrvatin S;Schuetz C;Bonal C;Rezania A;Melton DA
已经在体外从人多能干细胞分化的胰岛素表达细胞缺乏成熟β细胞的葡萄糖响应性特征。研究了小鼠中的β细胞成熟以找到能够筛选体外诱导真正β细胞的因子的遗传标记。我们发现功能性β细胞成熟的标志是胰岛素分泌的葡萄糖阈值增加和基因urocortin 3的表达。
Insulin expressing cells that have been differentiated from human pluripotent stem cells in vitro lack the glucose responsiveness characteristic of mature β-cells. β-cell maturation in mice was studied to find genetic markers that enable screens for factors that induce bona fide β-cells in vitro. We find that functional β-cell maturation is marked by an increase in the glucose threshold for insulin secretion and by expression of the gene urocortin 3.