Toward Biocompatible Nuclear Hyperpolarization Using Signal Amplification by Reversible Exchange: Quantitative in Situ Spectroscopy and High-Field Imaging
Toward Biocompatible Nuclear Hyperpolarization Using Signal Amplification by Reversible Exchange: Quantitative in Situ Spectroscopy and High-Field Imaging
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DOI：
10.1021/ac403653q
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发表时间：
2014-02-04
影响因子：
7.4
通讯作者：
von Elverfeldtt, Dominik
中科院分区：
文献类型：
--
作者：
Hoevener, Jan-Bernd;Schwaderlapp, Niels;von Elverfeldtt, Dominik
通过底物和仲氢在催化中心的可逆交换（SABRE）的信号放大有望克服磁共振固有的不敏感性。为了将新方法应用于生物医学应用，需要开发实验设备、原位定量方法和生物相容性溶剂。我们目前的结果详细介绍了低场SABRE偏振器，它提供了良好的控制实验条件，定义的自旋操作，并允许在原位检测的热极化和超极化样品。本文介绍了一种利用热极化参比物对超极化产额进行原位绝对定量的方法。对于148 μ mol的物质，最大信噪比接近10（3），相对于SABRE的偏振转移场，信号增强10（6），或绝对H-1偏振级接近10（-2）。在迈向生物医学应用的重要一步中，我们使用纯的超极化吡啶（d（3））和C-13烟酰胺和11%乙醇水溶液演示了H-1原位NMR以及H-1和C-13高场MRI。进一步增加超极化产额，在原位检测的影响，并在体内应用进行了讨论。
Signal amplification by reversible exchange (SABRE) of a substrate and parahydrogen at a catalytic center promises to overcome the inherent insensitivity of magnetic resonance. In order to apply the new approach to biomedical applications, there is a need to develop experimental equipment, in situ quantification methods, and a biocompatible solvent. We present results detailing a low-field SABRE polarizer which provides well-controlled experimental conditions, defined spins manipulations, and which allows in situ detection of thermally polarized and hyperpolarized samples. We introduce a method for absolute quantification of hyperpolarization yield in situ by means of a thermally polarized reference. A maximum signal-to-noise ratio of similar to 10(3) for 148 mu mol of substance, a signal enhancement of 10(6) with respect to polarization transfer field of SABRE, or an absolute H-1-polarization level of approximate to 10(-2) is achieved. In an important step toward biomedical application, we demonstrate H-1 in situ NMR as well as H-1 and C-13 high-field MRI using hyperpolarized pyridine (d(3)) and C-13 nicotinamide in pure and 11% ethanol in aqueous solution. Further increase of hyperpolarization yield, implications of in situ detection, and in vivo application are discussed.