The pH-responsive transcription factor PacC regulates mycelial growth, fruiting body development, and ganoderic acid biosynthesis in Ganoderma lucidum
The pH-responsive transcription factor PacC regulates mycelial growth, fruiting body development, and ganoderic acid biosynthesis in Ganoderma lucidum
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pH响应转录因子PacC调节灵芝菌丝生长、子实体发育和灵芝酸生物合成
DOI：
10.3852/16-079
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发表时间：
2016-11-01
期刊：
影响因子：
2.8
通讯作者：
Zhao, Ming-Wen
中科院分区：
文献类型：
--
作者：
Wu, Feng-Li;Zhang, Guang;Zhao, Ming-Wen
摘要 灵芝是一种广泛应用于中药的药用大型真菌。尽管如此，对该生物体基础生物学研究的缺乏阻碍了其商业价值的进一步开发。 pH 响应转录因子 PacC/Rim101 控制着许多真菌对环境 pH 的适应、发育和次生代谢。在这项研究中，在高等担子菌灵芝中鉴定出了编码 GlPacC 的 PacC/Rim101 同源物。 GlPacC 由 807 个氨基酸组成，包含三个典型的 C2H2 锌指结构域、两个潜在的 PEST 结构域、一个推定的 PKA 磷酸化位点和一个推定的核定位信号 (NLS)。当真菌处于中性和碱性条件下时，GlPacC 转录水平较高，GlPacC 的沉默会损害真菌对环境 pH 的反应。在 GlPacC 沉默菌株中，菌丝分支（营养菌丝和气生菌丝）之间的距离显着增加。 GlPacC沉默的菌株在固体培养基上生长异常或病态，并且不能形成原基和子实体。在GlPacC沉默菌株中，灵芝酸含量、ss和ls转录本的水平以及代谢中间体角鲨烯和羊毛甾醇的含量均上调。我们的结果表明，GlPacC 具有功能性，并且在灵芝菌丝生长、子实体发育和灵芝酸生物合成中发挥着复杂的作用。
Abstract Ganoderma lucidum is a medicinal macrofungus that is widely used in traditional Chinese medicine. Nonetheless, the scarcity of basic biological studies of this organism has hindered the further development of its commercial value. The pH-responsive transcription factor PacC/Rim101 governs the adaptation to environmental pH, the development and the secondary metabolism of many fungi. In this study, a homologue of PacC/Rim101 that encodes GlPacC was identified in the higher basidiomycete G. lucidum. GlPacC is composed of 807 amino acids and contains three typical C2H2 zinc-finger domains, two potential PEST domains, a putative PKA phosphorylation site, and a putative nuclear localization signal (NLS). GlPacC was transcribed at a high level when the fungus was under neutral and alkaline conditions, and silencing of GlPacC impaired the fungal response to ambient pH. The distance between the hyphal branches (of vegetative hyphae and aerial hyphae) was significantly increased in the GlPacC-silenced strains. The GlPacC-silenced strains grew abnormally or became sickly on solid culture medium and were unable to form primordia and fruiting bodies. The ganoderic acid content, levels of the sqs and ls transcripts, and contents of the metabolic intermediates squalene and lanosterol were all up-regulated in the GlPacC-silenced strains. Our results indicate that GlPacC is functional and plays complex roles in mycelial growth, fruiting body development and ganoderic acid biosynthesis in G. lucidum.