Potential transmission opportunity of CTX-M-producing Escherichia coli on a large-scale chicken farm in Vietnam.
Potential transmission opportunity of CTX-M-producing Escherichia coli on a large-scale chicken farm in Vietnam.
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DOI：
10.1016/j.jgar.2017.09.014
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发表时间：
2017-10
影响因子：
4.6
通讯作者：
Thi Kim Ngan Bui;T. Bui;Shuhei Ueda;D. Le;Yoshimasa Yamamoto;I. Hirai
中科院分区：
文献类型：
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作者：
Thi Kim Ngan Bui;T. Bui;Shuhei Ueda;D. Le;Yoshimasa Yamamoto;I. Hirai
目的产超广谱β-内酰胺酶（ESBL）大肠埃希菌在世界范围内广泛传播。本研究调查了blaCTX-M阳性E.大肠杆菌的感染率和分子特征是否存在差异。从大型养鸡场的24个人和38只鸡以及社区的51个人和36只鸡中收集粪便样品。所有样本均于2013年6月至2014年6月期间在越南红河三角洲地区的巴维省采集。CTX-M-生产性E.用常规分型方法对分离株进行了大肠杆菌和遗传相关性分析。采用纸片扩散法对分离菌株进行药敏试验。养殖场工人、养鸡场鸡、社区个体和社区散养鸡的大肠杆菌检出率分别为83.3%、71.1%、54.9%和13.9%。平均而言，blaCTX-M阳性E.鸡源大肠杆菌对8.3种抗生素耐药。CTX-M阳性大肠杆菌中氨基糖苷类修饰酶基因的平均检出数（3.4个）和质粒介导的黏菌素耐药基因emcr-1的检出率（33.3%）均高于阴性大肠杆菌。与其他采样组相比，另外，6株CTX-M-65阳性大肠杆菌的脉冲场凝胶电泳（PFGE）图谱有两种类型。coli和CTX-M-55阳性E.结论鸡源性大肠埃希菌的传播机会较鸡源性大肠埃希菌更频繁。越南大型养鸡场colion。
ObjectivesExtended-spectrum β-lactamase (ESBL)-producingEscherichia colihave disseminated worldwide. This study investigatedblaCTX-M-positiveE. colion a large-scale Vietnamese chicken farm and analysed whether there was any difference in prevalence and molecular characteristics ofblaCTX-M-positiveE. colibetween the farm and the Vietnamese community.MethodsFaecal samples were collected from 24 human individuals and 38 chickens from the large-scale chicken farm as well as 51 humans and 36 chickens from the community. All samples were collected between June 2013 and June 2014 in Bavi Province in the Red River Delta region of Vietnam. Molecular characterisation of CTX-M-producingE. coliand genetic relatedness among the isolates were evaluated by conventional typing methods. Antimicrobial susceptibility of the isolates was evaluated by the disk diffusion method.ResultsThe prevalence ofblaCTX-M-positiveE. coliwas 83.3%, 71.1%, 54.9% and 13.9% in farm workers, farm chickens, community individuals and community backyard chickens, respectively. On average,blaCTX-M-positiveE. coliisolates from farm chickens were resistant to 8.3 different antibiotics. The average number of detected aminoglycoside-modifying enzyme genes (3.4 genes) and the detection rate of the plasmid-mediated colistin resistance genemcr-1(33.3%) were higher inblaCTX-M-positiveE. coliisolated from farm chickens compared with other sampling groups. In addition, two types of indistinguishable pulsed-field gel electrophoresis (PFGE) patterns were observed in sixblaCTX-M-65-positiveE. coliand threeblaCTX-M-55-positiveE. colifrom farm chickens.ConclusionsThese results suggest a more frequent transmission opportunity ofblaCTX-M-positiveE. colion the large-scale Vietnamese chicken farm.