A bioinformatic analysis of zinc transporters in intestinal Lactobacillaceae.
A bioinformatic analysis of zinc transporters in intestinal Lactobacillaceae.
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肠道乳杆菌科锌转运蛋白的生物信息分析。
DOI：
10.1093/mtomcs/mfad044
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Zastrow,MelissaL
中科院分区：
文献类型：
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作者：
Huynh,Uyen;Nguyen,HazelN;Trinh,BrittanyK;Elhaj,Joanna;Zastrow,MelissaL
作为第二丰富的过渡元素和许多蛋白质的关键辅因子，锌对所有生物体的生存至关重要。为了维持所需的锌水平并防止毒性过载，细胞和生物体具有用于锌的摄取和流出的金属转运蛋白的集合。在细菌中，金属转运蛋白对于模式生物和许多病原体都有很好的定义，但很少有研究探索来自肠道微生物群的细菌中的金属转运蛋白，包括锌转运蛋白。健康的人类肠道微生物群包括数百种物种，其中，来自乳杆菌科的细菌被充分证明对健康具有各种有益作用。此外，饮食中金属摄入量的变化，如锌和铁，经常与乳酸杆菌科丰度的变化相关。然而，很少有研究探讨锌的需求和锌的稳态机制，乳酸杆菌科。在这里，我们应用生物信息学的方法，以确定和比较预测的锌吸收和流出蛋白质在几个Lactobacillaceae属的肠道相关性。从UniProt数据库中检索到的蛋白质组中，目前很少有乳酸杆菌具有锌转运蛋白，但基于蛋白质序列的同源性搜索显示，高亲和力ABC转运蛋白基因可能很常见，尽管具有属特异性结构域特征。P型ATP酶转运蛋白可能也很常见，一些乳酸杆菌属编码预测的锌外排阳离子扩散促进剂。这一分析证实了乳酸杆菌携带多种锌转运蛋白同源物的基因，并为阐明这些细菌中锌稳态机制的系统实验研究提供了基础。
As the second most abundant transition element and a crucial cofactor for many proteins, zinc is essential for the survival of all living organisms. To maintain required zinc levels and prevent toxic overload, cells and organisms have a collection of metal transport proteins for uptake and efflux of zinc. In bacteria, metal transport proteins are well defined for model organisms and many pathogens, but fewer studies have explored metal transport proteins, including those for zinc, in commensal bacteria from the gut microbiota. The healthy human gut microbiota comprises hundreds of species and among these, bacteria from theLactobacillaceaefamily are well documented to have various beneficial effects on health. Furthermore, changes in dietary metal intake, such as for zinc and iron, are frequently correlated with changes in abundance ofLactobacillaceae. Few studies have explored zinc requirements and zinc homeostasis mechanisms inLactobacillaceae, however. Here we applied a bioinformatics approach to identify and compare predicted zinc uptake and efflux proteins in severalLactobacillaceaegenera of intestinal relevance. FewLactobacillaceaehad zinc transporters currently annotated in proteomes retrieved from the UniProt database, but protein sequence-based homology searches revealed that high-affinity ABC transporter genes are likely common, albeit with genus-specific domain features. P-type ATPase transporters are probably also common and someLactobacillaceaegenera code for predicted zinc efflux cation diffusion facilitators. This analysis confirms thatLactobacillaceaeharbor genes for various zinc transporter homologs, and provides a foundation for systematic experimental studies to elucidate zinc homeostasis mechanisms in these bacteria.