Subtyping of influenza neuraminidase using mass spectrometry
Subtyping of influenza neuraminidase using mass spectrometry
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DOI：
10.1039/c3an00086a
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Downard, Kevin M.
中科院分区：
文献类型：
--
作者：
Nguyen, An P.;Downard, Kevin M.
采用高分辨率质谱法的蛋白质分型方法被证明能够区分感染人类和动物的A型流感病毒的所有九种神经氨酸酶亚型。通过对人类和动物宿主中所有h1n1 - n9亚型流感神经氨酸酶序列的比对，确定了色氨酸肽中的保守序列。那些在质量上是唯一的代表特征肽，当在流感神经氨酸酶或整个病毒消化的质谱中检测到这些特征肽时，使菌株能够可靠地分型。还证明了区分源自人H5N1和H1N1毒株的N1神经氨酸酶的能力。与传统的基于分子的PCR方法相比，该方法提供了一种更快速和直接的方法来确定病毒的亚型，具有相当的灵敏度。这应有助于在发生地方流行病或全球大流行病时更迅速地作出反应。
A proteotyping approach which employs high resolution mass spectrometry is shown to be able to differentiate all nine neuraminidase subtypes of type A influenza viruses that infect both humans and animals. Conserved sequences among tryptic peptides were identified through alignments of influenza neuraminidase sequences across all subtypes N1-N9 among human and animal hosts. Those that were unique in mass represent signature peptides which, when detected in the mass spectra of an influenza neuraminidase or whole virus digest, enable strains to be subtyped with confidence. The ability to distinguish N1 neuraminidase derived from human H5N1 and H1N1 strains is also demonstrated. The approach provides a more rapid and direct approach with which to subtype the virus than conventional molecular based PCR methods with comparable sensitivity. This should help facilitate a more rapid response in the event of a local epidemic or global pandemic.