Confocal imaging of early heart development in Xenopus laevis
Confocal imaging of early heart development in Xenopus laevis
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DOI：
10.1006/dbio.1999.9558
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发表时间：
2000-02-01
影响因子：
2.7
通讯作者：
Weeks, DL
中科院分区：
文献类型：
--
作者：
Kolker, SJ;Tajchman, U;Weeks, DL
非洲爪哇为心血管发育研究提供了许多有利条件。胚胎相当大，很容易获得，可以在环境温度下在简单的缓冲液中发育。尽管存在心脏发育的经典描述，但使用整体免疫组织化学方法和共聚焦显微镜的能力可能会增强理解正常和实验扰动的心血管发育的能力。我们已经开始研究非洲爪哇心脏发育的早期阶段，寻求识别抗体和固定剂，以便轻松检查正在发育的心脏。我们使用抗牛心肌肌钙蛋白T和鸡原肌球蛋白的单抗(MAbb)来显示心肌，用识别牛VI型胶原的山羊抗体来染色血管衬里，用抗鸡原纤维蛋白的JB3单抗来显示早期发育中的各种心血管组织。给出了胚胎阶段24-46的结果。(C)2000年学术出版社。
Xenopus laevis provides a number of advantages to studies on cardiovascular development. The embryos are fairly large, are easy to obtain, and can develop at ambient temperature in simple buffer solutions. Although classic descriptions of heart development exist, the ability to use whole-mount immunohistochemical methods and confocal microscopy may enhance the ability to understand both normal and experimentally perturbed cardiovascular development. We have started to examine the early stages of cardiac development in Xenopus, seeking to identify antibodies and fixatives that allow easy examination of the developing heart. We have used monoclonal antibodies (mAbs) raised against bovine cardiac troponin T and chicken tropomyosin to visualize cardiac muscle, a goat antibody recognizing bovine type VI collagen to stain the lining of vessels, and the JB3 mAb raised against chicken fibrillin, which allows the visualization of a variety of cardiovascular tissues during early development. Results from embryonic stages 24-46 are presented. (C) 2000 Academic Press.