On the participation of mTOR in recognition memory
On the participation of mTOR in recognition memory
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DOI：
10.1016/j.nlm.2007.10.002
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发表时间：
2008-03-01
影响因子：
2.7
通讯作者：
Cammarota, Martin
中科院分区：
文献类型：
--
作者：
Myskiw, Jociane C.;Rossato, Janine I.;Cammarota, Martin
有证据表明，大脑中与记忆相关的区域需要激活神经元蛋白质合成机制，以巩固和保持助记痕迹。在这里，我们报告了海马mTOR（一种参与mRNA翻译起始的蛋白激酶）的抑制，在训练后立即或180分钟而不是540分钟损害了长期物体识别记忆的巩固，而不影响短期记忆保留或探索行为。当长期记忆再激活后，在熟悉的物体存在下，将mTOR抑制剂雷帕霉素（RAP）输注到背侧CA1中，对记忆的保留没有影响。然而，当动物接触到一个新的和一个熟悉的物体后立即给予RAP时，它们的记忆都会受损。在没有其他刺激的情况下，在暴露于两个新物体或探索训练场地后，RAP的失忆效果是持久的，这表明它是偶然的，在存在行为相关和新线索的情况下，巩固痕迹的重新激活。我们的研究结果表明，mTOR的活性在海马背侧是巩固物体识别记忆所必需的，并且表明在存在一组特定线索的情况下，记忆检索后抑制该激酶会阻碍原始识别记忆痕迹的持久性。(C) 2007爱思唯尔公司版权所有。
Evidence indicates that activation of the neuronal protein synthesis machinery is required in areas of the brain relevant to memory for consolidation and persistence of the mnemonic trace. Here, we report that inhibition of hippocampal mTOR, a protein kinase involved in the initiation of mRNA translation, immediately or 180 min but not 540 min after training impairs consolidation of long-term object recognition memory without affecting short-term memory retention or exploratory behavior. When infused into dorsal CA1 after long-term memory reactivation in the presence of familiar objects the mTOR inhibitor rapamycin (RAP) did not affect retention. However, when given immediately after exposing animals to a novel and a familiar object, RAP impaired memory for both of them. The amnesic effect of the post-retrieval administration of RAP was long-lasting, did not happen after exposure to two novel objects or following exploration of the training arena in the absence of other stimuli, suggesting that it was contingent with reactivation of the consolidated trace in the presence of a behaviorally relevant and novel cue. Our results indicate that mTOR activity is required in the dorsal hippocampus for consolidation of object recognition memory and suggest that inhibition of this kinase after memory retrieval in the presence of a particular set of cues hinders persistence of the original recognition memory trace. (C) 2007 Elsevier Inc. All rights reserved.