A mutation inactivating the methyltransferase gene in avirulent Madrid E strain of Rickettsia prowazekii reverted to wild type in the virulent revertant strain Evir.
A mutation inactivating the methyltransferase gene in avirulent Madrid E strain of Rickettsia prowazekii reverted to wild type in the virulent revertant strain Evir.
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普瓦泽基立克次体无毒马德里E菌株甲基转移酶基因失活的突变在强毒回复菌株Evir中恢复为野生型。
DOI：
10.1016/j.vaccine.2005.11.044
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Yu,Xue-Jie
中科院分区：
文献类型：
--
作者：
Zhang,Jian-Zhi;Hao,Jun-Fang;Walker,DavidH;Yu,Xue-Jie
普罗瓦泽基立克次体马德里 E (E) 株是一种有效的疫苗，但在动物中传代时可恢复毒性状态。本研究的目的是通过比较E株及其强毒回复Evir株的遗传结构，鉴定可能决定R. prowazekii毒力的回复突变。我们确定编码甲基转移酶的基因（Rp028/Rp027）在无毒力E株中发生移码突变，但在强毒回复性Evir株和野生型强毒Breinl株中没有发生移码突变。我们得出的结论是，E 株基因突变在强毒回复 Evir 株中恢复为野生型。该突变是否在E株减毒中发挥重要作用还需要进一步研究。
Rickettsia prowazekii Madrid E (E) strain is an effective vaccine, but can revert to virulent status when passaged in animals. The aim of this study is to identify the reverse mutation that may determine the virulence of R. prowazekii by comparing the genetic structures of E strain and its virulent revertant Evir strain. We determined that the gene (Rp028/Rp027) encoding the methyltransferase was mutated by frameshift in avirulent E strain but not in virulent revertant Evir strain and wild type virulent Breinl strain. We conclude that the mutation in the E strain gene reverts to wild type in the virulent revertant Evir strain. Whether the mutation plays an essential role in the attenuation of E strain needs to be further investigated.