The propionic acid and butyric acid in serum but not in feces are increased in patients with diarrhea-predominant irritable bowel syndrome
The propionic acid and butyric acid in serum but not in feces are increased in patients with diarrhea-predominant irritable bowel syndrome
复制标题
DOI：
10.1186/s12876-020-01212-3
复制
发表时间：
2020-03-16
影响因子：
2.4
通讯作者：
Xiong, Lishou
中科院分区：
文献类型：
--
作者：
Tian, Zhenyi;Zhuang, Xiaojun;Xiong, Lishou
背景 短链脂肪酸（SCFA）改变已被报道在肠易激综合征（IBS）中，但结果是相互矛盾的。我们的研究旨在探讨腹泻型IBS（IBS-D）患者SCFAs的变化及其在IBS发生和发展中的潜在作用。方法 我们招募了罗马 IV 标准定义的 IBS-D 患者以及年龄和性别匹配的健康对照 (HC)。开发了顶空固相微萃取气相色谱-质谱（HS-SPME-GC-MS）方法，用于分析粪便和血清中的乙酸、丙酸和丁酸。结果 与HCs相比，IBS-D组血清丙酸盐（2.957±0.157 vs 2.843±0.098 mmol/L，P=0.012）和丁酸盐（2.798±0.126 vs 2.697±0.077 mmol/L，P=0.012）水平显着升高。两组粪便中乙酸盐（4.953 +/- 1.065 vs 4.774 +/- 1.465 mg/g，P = 0.679）、丙酸盐（6.342 +/- 1.005 vs 6.282 +/- 1.077 mg/g，P = 0.868）和丁酸盐（2.984 +/- 1.077 mg/g，P = 0.868）的浓度没有显着差异。 0.512 与 3.071 +/- 0.447 毫克/克，P = 0.607）。结论 IBS-D 患者血清中肠道菌群代谢物丙酸和丁酸增加，但粪便中不增加。提示丙酸、丁酸可能与IBS的发生、发展有关。
Background Short-chain fatty acids (SCFAs) alteration have been reported in irritable bowel syndrome (IBS), but the results are conflicting. Our study aims to explore the alteration of SCFAs in patients with diarrhea-predominant IBS (IBS-D) and their potential role in the occurrence and development of IBS. Methods We recruited patients with IBS-D defined by Rome IV criteria and age-and-gender matched healthy controls (HCs). A headspace solid-phase microextraction gas chromatography-mass spectrometric (HS-SPME-GC-MS) method was developed for the analysis of acetic, propionic and butyric acid in feces and serum. Results Compared with HCs, the levels of the serum propionate (2.957 +/- 0.157 vs 2.843 +/- 0.098 mmol/L, P = 0.012) and butyrate (2.798 +/- 0.126 vs 2.697 +/- 0.077 mmol/L, P = 0.012) were significantly higher in IBS-D group. No significant differences were found among two groups with regard to the concentration of fecal acetate (4.953 +/- 1.065 vs 4.774 +/- 1.465 mg/g, P = 0.679), propionate (6.342 +/- 1.005 vs 6.282 +/- 1.077 mg/g, P = 0.868) and butyrate (2.984 +/- 0.512 vs 3.071 +/- 0.447 mg/g, P = 0.607). Conclusions Metabolites of gut microbiota, the propionic and butyric acid, are increased in patients with IBS-D in serum but not in feces. It suggests that propionic and butyric acid might be associated with the occurrence and development of IBS.