rFN/Cad-11-Modified Collagen Type II Biomimetic Interface Promotes the Adhesion and Chondrogenic Differentiation of Mesenchymal Stem Cells
rFN/Cad-11-Modified Collagen Type II Biomimetic Interface Promotes the Adhesion and Chondrogenic Differentiation of Mesenchymal Stem Cells
复制标题
rFN/Cad-11修饰的II型胶原仿生界面促进间充质干细胞的粘附和软骨分化
DOI：
10.1089/ten.tea.2012.0447
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发表时间：
2013-11-01
影响因子：
4.1
通讯作者：
Fan, Yujiang
中科院分区：
文献类型：
--
作者：
Dong, Shiwu;Guo, Hongfeng;Fan, Yujiang
细胞-材料界面的性质是软骨组织工程细胞成功发挥功能的决定因素。目前，通常通过使用多肽来促进细胞粘附;然而，由于其缺乏互补或调节结构域，必须修饰多肽以提高其促进粘附的能力。在这项研究中，我们利用基于基质的仿生修饰的原理和重组蛋白，它跨越片段7-10的纤维连接蛋白模块III（异嗜性基序）和细胞外结构域1-2的钙粘蛋白-11（rFN/Cad-11）（同嗜性基序），改性II型胶原（Col II）海绵的界面。我们表明，设计的材料是能够刺激细胞增殖和促进更好的软骨分化的兔间充质干细胞（MSCs）在体外比FN修饰的表面和阴性对照。此外，Col II/rFN/Cad-11-MSC复合物在体内刺激软骨形成;单独的Col II的软骨形成效果要不那么显著。这些结果表明，rFN/Cad-11修饰的II型胶原仿生界面具有促进粘附和刺激软骨分化的双重生物学功能。因此，这种物质可以作为软骨组织工程的理想支架材料，促进体内受损软骨的修复。
Properties of the cell-material interface are determining factors in the successful function of cells for cartilage tissue engineering. Currently, cell adhesion is commonly promoted through the use of polypeptides; however, due to their lack of complementary or modulatory domains, polypeptides must be modified to improve their ability to promote adhesion. In this study, we utilized the principle of matrix-based biomimetic modification and a recombinant protein, which spans fragments 7-10 of fibronectin module III (heterophilic motif) and extracellular domains 1-2 of cadherin-11 (rFN/Cad-11) (homophilic motif), to modify the interface of collagen type II (Col II) sponges. We showed that the designed material was able to stimulate cell proliferation and promote better chondrogenic differentiation of rabbit mesenchymal stem cells (MSCs) in vitro than both the FN modified surfaces and the negative control. Further, the Col II/rFN/Cad-11-MSCs composite stimulated cartilage formation in vivo; the chondrogenic effect of Col II alone was much less significant. These results suggested that the rFN/Cad-11-modified collagen type II biomimetic interface has dual biological functions of promoting adhesion and stimulating chondrogenic differentiation. This substance, thus, may serve as an ideal scaffold material for cartilage tissue engineering, enhancing repair of injured cartilage in vivo.