A microarray analysis of urinary microRNAs in renal diseases
A microarray analysis of urinary microRNAs in renal diseases
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DOI：
10.1007/s10157-013-0906-5
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发表时间：
2014-10-01
影响因子：
2.3
通讯作者：
Kubota, Isao
中科院分区：
文献类型：
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作者：
Konta, Tsuneo;Ichikawa, Kazunobu;Kubota, Isao
microRNAs（miRNAs）是一个内源性小分子非编码RNA家族，与肾脏疾病的发生发展密切相关。为了阐明尿中miRNAs（UmiRNAs）是否可用于肾脏疾病的评估，我们检测了各种肾脏疾病中UmiRNAs的表达谱。我们从5名健康对照和71名肾脏疾病患者的尿液标本中提取了miRNAs，我们通过微阵列分析检测了临床和组织学参数与UmiRNAs谱之间的相关性。疾病与健康对照组相比，其水平与尿蛋白和肾小球硬化程度相关。微阵列分析检测到83-137个不同的UmiRNAs。我们在健康对照和肾病患者中观察到80- 99%的miRNA。大多数UmiRNAs在肾脏疾病患者中显示出比健康对照更高的信号强度，包括39种miRNAs显示出比健康对照高100倍的信号强度。在每种类型的肾脏疾病中观察到不同的UmiRNAs模式。肾组织和UmiRNAs的比较显示，样本谱相似，信号强度显著相关，本研究表明UmiRNAs与肾脏病理变化相关，UmiRNAs谱呈现不同的模式，对应于肾脏疾病的类型。这些结果表明，UmiRNAs可能被用作肾脏疾病的新生物标志物。
MicroRNAs (miRNAs), a family of endogenous small non-coding RNAs, are associated with the development of renal diseases. To clarify whether urinary miRNAs (UmiRNAs) can be used for the evaluation of renal disease, we examined the profiles of UmiRNAs in various renal diseases.We extracted miRNAs from urine specimens of 5 healthy controls and 71 patients with renal diseases, and we examined the correlation between clinical and histological parameters and the profile of UmiRNAs by microarray analysis.The urinary concentration of miRNAs increased in patients with renal disease compared with healthy controls, and the levels correlated with urinary protein and the degree of glomerular sclerosis. The microarray analysis detected 83-137 distinct UmiRNAs. We observed 80-99 % of the miRNAs in both the healthy controls and the renal disease patients. The majority of UmiRNAs displayed higher signal intensity in renal disease patients than in healthy controls, including 39 miRNAs exhibiting signal intensities 100 times greater than in healthy controls. A different pattern of UmiRNAs was observed in each type of renal disease. A comparison of renal tissue and UmiRNAs revealed that the sample profiles were similar and that their signal intensity was significantly correlated.This study demonstrated that UmiRNAs are correlated with renal pathological changes and that the profile of UmiRNAs presented different patterns corresponding to the type of renal disease. These results suggest that UmiRNAs can potentially be used as novel biomarkers for renal diseases.